8j6h
From Proteopedia
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8j6h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8j6h OCA], [https://pdbe.org/8j6h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8j6h RCSB], [https://www.ebi.ac.uk/pdbsum/8j6h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8j6h ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8j6h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8j6h OCA], [https://pdbe.org/8j6h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8j6h RCSB], [https://www.ebi.ac.uk/pdbsum/8j6h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8j6h ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| - | == | + | <div style="background-color:#fffaf0;"> |
| - | + | == Publication Abstract from PubMed == | |
| + | The nucleoside inosine is a main intermediate of purine nucleotide catabolism in Saccharomyces cerevisiae and is produced via the dephosphorylation of inosine monophosphate (IMP) by IMP-specific 5'-nucleotidase 1 (ISN1), which is present in many eukaryotic organisms. Upon transition of yeast from oxidative to fermentative growth, ISN1 is important for intermediate inosine accumulation as purine storage, but details of ISN1 regulation are unknown. We characterized structural and kinetic behavior of ISN1 from S. cerevisiae (ScISN1) and showed that tetrameric ScISN1 is negatively regulated by inosine and adenosine triphosphate (ATP). Regulation involves an inosine-binding allosteric site along with IMP-induced local and global conformational changes in the monomer and a tetrameric re-arrangement, respectively. A proposed interaction network propagates local conformational changes in the active site to the intersubunit interface, modulating the allosteric features of ScISN1. Via ATP and inosine, ScISN1 activity is likely fine-tuned to regulate IMP and inosine homeostasis. These regulatory and catalytic features of ScISN1 contrast with those of the structurally homologous ISN1 from Plasmodium falciparum, indicating that ISN1 enzymes may serve different biological purposes in different organisms. | ||
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| + | Structure, cooperativity and inhibition of the inosine 5'-monophosphate-specific phosphatase from Saccharomyces cerevisiae.,Byun S, Park C, Suh JY, Witte CP, Rhee S FEBS J. 2024 May;291(9):1992-2008. doi: 10.1111/febs.17093. Epub 2024 Feb 16. PMID:38362806<ref>PMID:38362806</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 8j6h" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Current revision
Structure and allosteric regulation of the inosine 5'-monophosphate-specific phosphatase ISN1 from Saccharomyces cerevisiae
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