8ps2

From Proteopedia

(Difference between revisions)

Current revision

Asymmetric unit of the yeast fatty acid synthase with ACP at the enoyl reductase domain (FASam sample)

Structural highlights

8ps2 is a 3 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 2.9Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Fatty acids (FAs) play a central metabolic role in living cells as constituents of membranes, cellular energy reserves, and second messenger precursors. A 2.6 MDa FA synthase (FAS), where the enzymatic reactions and structures are known, is responsible for FA biosynthesis in yeast. Essential in the yeast FAS catalytic cycle is the acyl carrier protein (ACP) that actively shuttles substrates, biosynthetic intermediates, and products from one active site to another. We resolve the S. cerevisiae FAS structure at 1.9 A, elucidating cofactors and water networks involved in their recognition. Structural snapshots of ACP domains bound to various enzymatic domains allow the reconstruction of a full yeast FA biosynthesis cycle. The structural information suggests that each FAS functional unit could accommodate exogenous proteins to incorporate various enzymatic activities, and we show proof-of-concept experiments where ectopic proteins are used to modulate FAS product profiles.

Reconstruction of a fatty acid synthesis cycle from acyl carrier protein and cofactor structural snapshots.,Singh K, Bunzel G, Graf B, Yip KM, Neumann-Schaal M, Stark H, Chari A Cell. 2023 Nov 9;186(23):5054-5067.e16. doi: 10.1016/j.cell.2023.10.009. PMID:37949058^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Singh K, Bunzel G, Graf B, Yip KM, Neumann-Schaal M, Stark H, Chari A. Reconstruction of a fatty acid synthesis cycle from acyl carrier protein and cofactor structural snapshots. Cell. 2023 Nov 9;186(23):5054-5067.e16. PMID:37949058 doi:10.1016/j.cell.2023.10.009

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/8ps2"

@@ Line 8: / Line 8: @@
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ps2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ps2 OCA], [https://pdbe.org/8ps2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ps2 RCSB], [https://www.ebi.ac.uk/pdbsum/8ps2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ps2 ProSAT]</span></td></tr>
 </table>
-== Function ==
-[https://www.uniprot.org/uniprot/FAS1_YEAST FAS1_YEAST] Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. The beta subunit contains domains for: [acyl-carrier-protein] acetyltransferase and malonyltransferase, S-acyl fatty acid synthase thioesterase, enoyl-[acyl-carrier-protein] reductase, and 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==

8ps2

From Proteopedia

Current revision

Asymmetric unit of the yeast fatty acid synthase with ACP at the enoyl reductase domain (FASam sample)

Structural highlights

Publication Abstract from PubMed

References

Contents

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