8spu
From Proteopedia
(Difference between revisions)
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8spu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8spu OCA], [https://pdbe.org/8spu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8spu RCSB], [https://www.ebi.ac.uk/pdbsum/8spu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8spu ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8spu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8spu OCA], [https://pdbe.org/8spu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8spu RCSB], [https://www.ebi.ac.uk/pdbsum/8spu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8spu ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| - | == | + | <div style="background-color:#fffaf0;"> |
| - | + | == Publication Abstract from PubMed == | |
| + | Pioneer transcription factors are essential for cell fate changes by targeting closed chromatin. OCT4 is a crucial pioneer factor that can induce cell reprogramming. However, the structural basis of how pioneer factors recognize the in vivo nucleosomal DNA targets is unknown. Here, we determine the high-resolution structures of the nucleosome containing human LIN28B DNA and its complexes with the OCT4 DNA binding region. Three OCT4s bind the pre-positioned nucleosome by recognizing non-canonical DNA sequences. Two use their POUS domains while the other uses the POUS-loop-POUHD region; POUHD serves as a wedge to unwrap approximately 25 base pair DNA. Our analysis of previous genomic data and determination of the ESRRB-nucleosome-OCT4 structure confirmed the generality of these structural features. Moreover, biochemical studies suggest that multiple OCT4s cooperatively open the H1-condensed nucleosome array containing the LIN28B nucleosome. Thus, our study suggests a mechanism of how OCT4 can target the nucleosome and open closed chromatin. | ||
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| + | Structural mechanism of LIN28B nucleosome targeting by OCT4.,Guan R, Lian T, Zhou BR, Wheeler D, Bai Y Mol Cell. 2023 Jun 15;83(12):1970-1982.e6. doi: 10.1016/j.molcel.2023.05.030. PMID:37327775<ref>PMID:37327775</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 8spu" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Bai Y]] |
| - | [[Category: | + | [[Category: Guan R]] |
| - | [[Category: | + | [[Category: Lian T]] |
Current revision
Structure of ESRRB nucleosome bound OCT4 at site c
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