1x7q
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(New page: 200px<br /> <applet load="1x7q" size="450" color="white" frame="true" align="right" spinBox="true" caption="1x7q, resolution 1.45Å" /> '''Crystal structure o...)
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Revision as of 17:55, 12 November 2007
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Crystal structure of HLA-A*1101 with sars nucleocapsid peptide
Contents |
Overview
The structure of the human MHC-I molecule HLA-A*1101 in complex with a, nonameric peptide (KTFPPTEPK) has been determined by X-ray crystallography, to 1.45 A resolution. The peptide is derived from the SARS-CoV, nucleocapsid protein positions 362-370 (SNP362-370). It is conserved in, all known isolates of SARS-CoV and has been verified by in vitro, peptide-binding studies to be a good to intermediate binder to HLA-A*0301, and HLA-A*1101, with IC50 values of 70 and 186 nM, respectively, [Sylvester-Hvid et al. (2004), Tissue Antigens, 63, 395-400]. In terms of, the residues lining the peptide-binding groove, the HLA-A*1101-SNP362-370, complex is very similar to other known structures of HLA-A*1101 and, HLA-A*6801. The SNP362-370 peptide is held in place by 17 hydrogen bonds, to the alpha-chain residues and by nine water molecules which are also, tightly bound in the peptide-binding groove. Thr6 of the peptide (Thr6p), does not make efficient use of the middle (E) pocket. For vaccine, development, there seems to be a potential for optimization targeted at, this position. All residues except Thr2p and Lys9p are accessible for, T-cell recognition.
Disease
Known diseases associated with this structure: Abacavir hypersensitivity, susceptibility to OMIM:[142800], Ankylosing spondylitis, susceptibility to, 1 OMIM:[142800], Hypoproteinemia, hypercatabolic OMIM:[109700], Stevens-Johnson syndrome, susceptibility to OMIM:[142800]
About this Structure
1X7Q is a Protein complex structure of sequences from Homo sapiens with SO4 and GOL as ligands. Full crystallographic information is available from OCA.
Reference
High-resolution structure of HLA-A*1101 in complex with SARS nucleocapsid peptide., Blicher T, Kastrup JS, Buus S, Gajhede M, Acta Crystallogr D Biol Crystallogr. 2005 Aug;61(Pt 8):1031-40. Epub 2005, Jul 20. PMID:16041067
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