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| | <StructureSection load='6byd' size='340' side='right'caption='[[6byd]], [[Resolution|resolution]] 2.19Å' scene=''> | | <StructureSection load='6byd' size='340' side='right'caption='[[6byd]], [[Resolution|resolution]] 2.19Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6byd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BYD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BYD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6byd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BYD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BYD FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.195Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LAM4, LTC3, YHR080C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6byd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6byd OCA], [http://pdbe.org/6byd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6byd RCSB], [http://www.ebi.ac.uk/pdbsum/6byd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6byd ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6byd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6byd OCA], [https://pdbe.org/6byd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6byd RCSB], [https://www.ebi.ac.uk/pdbsum/6byd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6byd ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LAM4_YEAST LAM4_YEAST]] May be involved in sterol transfer between intracellular membranes.<ref>PMID:26001273</ref> | + | [https://www.uniprot.org/uniprot/LAM4_YEAST LAM4_YEAST] May be involved in sterol transfer between intracellular membranes.<ref>PMID:26001273</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Boudker, O]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Jentsch, J A]] | + | [[Category: Boudker O]] |
| - | [[Category: Kiburu, I N]] | + | [[Category: Jentsch JA]] |
| - | [[Category: Menon, A K]] | + | [[Category: Kiburu IN]] |
| - | [[Category: Pandey, K]] | + | [[Category: Menon AK]] |
| - | [[Category: Wu, J]] | + | [[Category: Pandey K]] |
| - | [[Category: Lipid transport]]
| + | [[Category: Wu J]] |
| - | [[Category: Sterol transport]]
| + | |
| Structural highlights
Function
LAM4_YEAST May be involved in sterol transfer between intracellular membranes.[1]
Publication Abstract from PubMed
The StARkin superfamily comprises proteins with steroidogenic acute regulatory protein related lipid transfer (StART) domains that are implicated in intracellular, non-vesicular lipid transport. A new family of membrane-anchored StARkins was recently identified, including six members Lam1-Lam6 in the yeast Saccharomyces cerevisiae. Lam1-Lam4 are anchored to the endoplasmic reticulum (ER) membrane at sites where the ER is tethered to the plasma membrane, and proposed to be involved in sterol homeostasis in yeast. To understand better the biological roles of these proteins, we carried out a structure-function analysis of the second StARkin domain of Lam4, here termed Lam4S2. NMR experiments indicated that Lam4S2 binds undergoes specific conformational changes upon binding sterol, and fluorescence-based assays revealed that it catalyzes sterol transport between vesicle populations in vitro, exhibiting a preference for vesicles containing anionic lipids. Using such vesicles, we found that sterols are transported at a rate of ~50 molecules per Lam4S2 per minute. Crystal structures of Lam4S2, with and without bound sterol revealed a largely hydrophobic but surprisingly accessible sterol binding pocket, with the 3-OH group of the sterol oriented towards its base. Single or multiple alanine or aspartic acid replacements of conserved lysine residues in a basic patch on the surface of Lam4S2 near the likely sterol entry/egress site strongly attenuated sterol transport. Our results suggest that Lam4S2 engages anionic membranes via a basic surface patch, enabling 'head-first' entry of sterol into the binding pocket followed by partial closure of the entryway. Reversal of these steps enables sterol egress.
Structural basis of sterol binding and transport by a yeast StARkin domain.,Jentsch JA, Kiburu I, Pandey K, Timme M, Ramlall T, Levkau B, Wu J, Eliezer D, Boudker O, Menon AK J Biol Chem. 2018 Feb 20. pii: RA118.001881. doi: 10.1074/jbc.RA118.001881. PMID:29463678[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gatta AT, Wong LH, Sere YY, Calderon-Norena DM, Cockcroft S, Menon AK, Levine TP. A new family of StART domain proteins at membrane contact sites has a role in ER-PM sterol transport. Elife. 2015 May 22;4. doi: 10.7554/eLife.07253. PMID:26001273 doi:http://dx.doi.org/10.7554/eLife.07253
- ↑ Jentsch JA, Kiburu I, Pandey K, Timme M, Ramlall T, Levkau B, Wu J, Eliezer D, Boudker O, Menon AK. Structural basis of sterol binding and transport by a yeast StARkin domain. J Biol Chem. 2018 Feb 20. pii: RA118.001881. doi: 10.1074/jbc.RA118.001881. PMID:29463678 doi:http://dx.doi.org/10.1074/jbc.RA118.001881
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