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| | <StructureSection load='6esv' size='340' side='right'caption='[[6esv]], [[Resolution|resolution]] 1.78Å' scene=''> | | <StructureSection load='6esv' size='340' side='right'caption='[[6esv]], [[Resolution|resolution]] 1.78Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6esv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhizobium_sp._nt-26 Rhizobium sp. nt-26]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ESV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ESV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6esv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudorhizobium_banfieldiae Pseudorhizobium banfieldiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ESV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ESV FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.78Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6esk|6esk]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6esv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6esv OCA], [https://pdbe.org/6esv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6esv RCSB], [https://www.ebi.ac.uk/pdbsum/6esv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6esv ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">aioX, NT26_p10026 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1125847 Rhizobium sp. NT-26])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6esv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6esv OCA], [http://pdbe.org/6esv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6esv RCSB], [http://www.ebi.ac.uk/pdbsum/6esv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6esv ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/L0NML6_9HYPH L0NML6_9HYPH] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Rhizobium sp. nt-26]] | + | [[Category: Pseudorhizobium banfieldiae]] |
| - | [[Category: Badilla, C]] | + | [[Category: Badilla C]] |
| - | [[Category: Cole, A]] | + | [[Category: Cole A]] |
| - | [[Category: Djordjevic, S]] | + | [[Category: Djordjevic S]] |
| - | [[Category: Santini, J]] | + | [[Category: Santini J]] |
| - | [[Category: Arsenic]]
| + | |
| - | [[Category: Arsenite-binding]]
| + | |
| - | [[Category: Periplasmic-binding protein]]
| + | |
| - | [[Category: Rhizobium nt-26]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| Structural highlights
Function
L0NML6_9HYPH
Publication Abstract from PubMed
Arsenic contamination of drinking water affects more than 140 million people worldwide. While toxic to humans, inorganic forms of arsenic (arsenite and arsenate), can be used as energy sources for microbial respiration. AioX and its orthologues (ArxX and ArrX) represent the first members of a new sub-family of periplasmic-binding proteins that serve as the first component of a signal transduction system, that's role is to positively regulate expression of arsenic metabolism enzymes. As determined by X-ray crystallography for AioX, arsenite binding only requires subtle conformational changes in protein structure, providing insights into protein-ligand interactions. The binding pocket of all orthologues is conserved but this alone is not sufficient for oxyanion selectivity, with proteins selectively binding either arsenite or arsenate. Phylogenetic evidence, clearly demonstrates that the regulatory proteins evolved together early in prokaryotic evolution and had a separate origin from the metabolic enzymes whose expression they regulate.
A new family of periplasmic-binding proteins that sense arsenic oxyanions.,Badilla C, Osborne TH, Cole A, Watson C, Djordjevic S, Santini JM Sci Rep. 2018 Apr 19;8(1):6282. doi: 10.1038/s41598-018-24591-w. PMID:29674678[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Badilla C, Osborne TH, Cole A, Watson C, Djordjevic S, Santini JM. A new family of periplasmic-binding proteins that sense arsenic oxyanions. Sci Rep. 2018 Apr 19;8(1):6282. doi: 10.1038/s41598-018-24591-w. PMID:29674678 doi:http://dx.doi.org/10.1038/s41598-018-24591-w
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