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| | <StructureSection load='6fej' size='340' side='right'caption='[[6fej]], [[Resolution|resolution]] 2.75Å' scene=''> | | <StructureSection load='6fej' size='340' side='right'caption='[[6fej]], [[Resolution|resolution]] 2.75Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6fej]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Anabaena_7120 Anabaena 7120]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FEJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FEJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6fej]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_7120_=_FACHB-418 Nostoc sp. PCC 7120 = FACHB-418]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FEJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6FEJ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=URE:UREA'>URE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">all4940 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=103690 Anabaena 7120])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=URE:UREA'>URE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fej FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fej OCA], [http://pdbe.org/6fej PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fej RCSB], [http://www.ebi.ac.uk/pdbsum/6fej PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fej ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6fej FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fej OCA], [https://pdbe.org/6fej PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6fej RCSB], [https://www.ebi.ac.uk/pdbsum/6fej PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6fej ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8YMJ3_NOSS1 Q8YMJ3_NOSS1] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Anabaena 7120]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Adir, N]] | + | [[Category: Nostoc sp. PCC 7120 = FACHB-418]] |
| - | [[Category: Harris, D]] | + | [[Category: Adir N]] |
| - | [[Category: Kirilovsky, D]] | + | [[Category: Harris D]] |
| - | [[Category: Muzzopappa, F]] | + | [[Category: Kirilovsky D]] |
| - | [[Category: Wilson, A]] | + | [[Category: Muzzopappa F]] |
| - | [[Category: Carotene cyanobacteria photoprotection urea]]
| + | [[Category: Wilson A]] |
| - | [[Category: Photosynthesis]]
| + | |
| Structural highlights
Function
Q8YMJ3_NOSS1
Publication Abstract from PubMed
A recently reported family of soluble cyanobacterial carotenoproteins, homologs of the C-terminal domain (CTDH) of the photoprotective Orange Carotenoid Protein, is suggested to mediate carotenoid transfer from the thylakoid membrane to the Helical Carotenoid Proteins, which are paralogs of the N-terminal domain of the OCP. Here we present the three-dimensional structure of a carotenoid-free CTDH variant from Anabaena (Nostoc) PCC 7120. This CTDH contains a cysteine residue at position 103. Two dimer-forming interfaces were identified, one stabilized by a disulfide bond between monomers and the second between each monomer's beta-sheets, both compatible with small-angle X-ray scattering data and likely representing intermediates of carotenoid transfer processes. The crystal structure revealed a major positional change of the C-terminal tail. Further mutational analysis revealed the importance of the C-terminal tail in both carotenoid uptake and delivery. These results have allowed us to suggest a detailed model for carotenoid transfer via these soluble proteins.
Structural rearrangements in the C-terminal domain homolog of Orange Carotenoid Protein are crucial for carotenoid transfer.,Harris D, Wilson A, Muzzopappa F, Sluchanko NN, Friedrich T, Maksimov EG, Kirilovsky D, Adir N Commun Biol. 2018 Aug 27;1:125. doi: 10.1038/s42003-018-0132-5. eCollection 2018. PMID:30272005[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Harris D, Wilson A, Muzzopappa F, Sluchanko NN, Friedrich T, Maksimov EG, Kirilovsky D, Adir N. Structural rearrangements in the C-terminal domain homolog of Orange Carotenoid Protein are crucial for carotenoid transfer. Commun Biol. 2018 Aug 27;1:125. doi: 10.1038/s42003-018-0132-5. eCollection 2018. PMID:30272005 doi:http://dx.doi.org/10.1038/s42003-018-0132-5
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