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| | <StructureSection load='6gw3' size='340' side='right'caption='[[6gw3]], [[Resolution|resolution]] 1.39Å' scene=''> | | <StructureSection load='6gw3' size='340' side='right'caption='[[6gw3]], [[Resolution|resolution]] 1.39Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6gw3]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GW3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GW3 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6gw3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Cannabis_sativa Cannabis sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GW3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GW3 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.39Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3,5,7-trioxododecanoyl-CoA_synthase 3,5,7-trioxododecanoyl-CoA synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.206 2.3.1.206] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gw3 OCA], [https://pdbe.org/6gw3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gw3 RCSB], [https://www.ebi.ac.uk/pdbsum/6gw3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gw3 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gw3 OCA], [http://pdbe.org/6gw3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gw3 RCSB], [http://www.ebi.ac.uk/pdbsum/6gw3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gw3 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/OLIS_CANSA OLIS_CANSA]] Polyketide synthase responsible for olivetol biosynthesis. Catalyzes the first step in the cannabinoids biosynthetic pathway. The preferred substrate is hexanoyl-CoA, but accepts also CoA esters with C4 to C8 aliphatic side chains. When using malonyl-CoA and hexanoyl-CoA as substrates, produces undetermined compounds distinct form olivetol or olivetolic acid (PubMed:19581347) that could be hexanoyl triacetic acid lactone (HTAL) (PubMed:18323613) and pentyl diacetic acid lactone (PDAL) (PubMed:22802619). Produces olivetolic acid when acting in concert with olivetolic acid cyclase (OAC).<ref>PMID:18323613</ref> <ref>PMID:19454282</ref> <ref>PMID:19581347</ref> <ref>PMID:22802619</ref> | + | [https://www.uniprot.org/uniprot/OLIS_CANSA OLIS_CANSA] Polyketide synthase responsible for olivetol biosynthesis. Catalyzes the first step in the cannabinoids biosynthetic pathway. The preferred substrate is hexanoyl-CoA, but accepts also CoA esters with C4 to C8 aliphatic side chains. When using malonyl-CoA and hexanoyl-CoA as substrates, produces undetermined compounds distinct form olivetol or olivetolic acid (PubMed:19581347) that could be hexanoyl triacetic acid lactone (HTAL) (PubMed:18323613) and pentyl diacetic acid lactone (PDAL) (PubMed:22802619). Produces olivetolic acid when acting in concert with olivetolic acid cyclase (OAC).<ref>PMID:18323613</ref> <ref>PMID:19454282</ref> <ref>PMID:19581347</ref> <ref>PMID:22802619</ref> |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: 3,5,7-trioxododecanoyl-CoA synthase]] | + | [[Category: Cannabis sativa]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Karuppiah, V]] | + | [[Category: Karuppiah V]] |
| - | [[Category: Leys, D]] | + | [[Category: Leys D]] |
| - | [[Category: 7-trioxododecanoyl-coa synthase]]
| + | |
| - | [[Category: Ligase]]
| + | |
| - | [[Category: Pks type iii]]
| + | |
| - | [[Category: Polyketide synthase]]
| + | |
| Structural highlights
Function
OLIS_CANSA Polyketide synthase responsible for olivetol biosynthesis. Catalyzes the first step in the cannabinoids biosynthetic pathway. The preferred substrate is hexanoyl-CoA, but accepts also CoA esters with C4 to C8 aliphatic side chains. When using malonyl-CoA and hexanoyl-CoA as substrates, produces undetermined compounds distinct form olivetol or olivetolic acid (PubMed:19581347) that could be hexanoyl triacetic acid lactone (HTAL) (PubMed:18323613) and pentyl diacetic acid lactone (PDAL) (PubMed:22802619). Produces olivetolic acid when acting in concert with olivetolic acid cyclase (OAC).[1] [2] [3] [4]
References
- ↑ Taguchi C, Taura F, Tamada T, Shoyama Y, Shoyama Y, Tanaka H, Kuroki R, Morimoto S. Crystallization and preliminary X-ray diffraction studies of polyketide synthase-1 (PKS-1) from Cannabis sativa. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Mar 1;64(Pt 3):217-20., doi: 10.1107/S1744309108003795. Epub 2008 Feb 29. PMID:18323613 doi:http://dx.doi.org/10.1107/S1744309108003795
- ↑ Taura F, Tanaka S, Taguchi C, Fukamizu T, Tanaka H, Shoyama Y, Morimoto S. Characterization of olivetol synthase, a polyketide synthase putatively involved in cannabinoid biosynthetic pathway. FEBS Lett. 2009 Jun 18;583(12):2061-6. doi: 10.1016/j.febslet.2009.05.024. Epub, 2009 May 19. PMID:19454282 doi:http://dx.doi.org/10.1016/j.febslet.2009.05.024
- ↑ Marks MD, Tian L, Wenger JP, Omburo SN, Soto-Fuentes W, He J, Gang DR, Weiblen GD, Dixon RA. Identification of candidate genes affecting Delta9-tetrahydrocannabinol biosynthesis in Cannabis sativa. J Exp Bot. 2009;60(13):3715-26. doi: 10.1093/jxb/erp210. Epub 2009 Jul 6. PMID:19581347 doi:http://dx.doi.org/10.1093/jxb/erp210
- ↑ Gagne SJ, Stout JM, Liu E, Boubakir Z, Clark SM, Page JE. Identification of olivetolic acid cyclase from Cannabis sativa reveals a unique catalytic route to plant polyketides. Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12811-6. doi:, 10.1073/pnas.1200330109. Epub 2012 Jul 16. PMID:22802619 doi:http://dx.doi.org/10.1073/pnas.1200330109
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