6h3v

From Proteopedia

(Difference between revisions)

Current revision

Bunyamwera Virus Glycoprotein Gc Head Domain

Structural highlights

6h3v is a 1 chain structure with sequence from Bunyamwera virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.9Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GP_BUNYW Glycoprotein N and Glycoprotein C interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion (By similarity). Non-structural protein M: Plays a role virion budding at Golgi tubes and in subcellular location of Glycoprotein C protein.^[1]

Publication Abstract from PubMed

Orthobunyaviruses (OBVs) form a distinct genus of arthropod-borne bunyaviruses that can cause severe disease upon zoonotic transmission to humans. Antigenic drift or genome segment re-assortment have in the past resulted in new pathogenic OBVs, making them potential candidates for causing emerging zoonoses in the future. Low-resolution electron cryo-tomography studies have shown that OBV particles feature prominent trimeric spikes, but their molecular organization remained unknown. Here we report X-ray crystallography studies of four different OBVs showing that the spikes are formed by an N-terminal extension of the fusion glycoprotein Gc. Using Schmallenberg virus, a recently emerged OBV, we also show that the projecting spike is the major target of the neutralizing antibody response, and provide X-ray structures in complex with two protecting antibodies. We further show that immunization of mice with the spike domains elicits virtually sterilizing immunity, providing fundamental knowledge essential in the preparation for potential newly emerging OBV zoonoses.

Orthobunyavirus spike architecture and recognition by neutralizing antibodies.,Hellert J, Aebischer A, Wernike K, Haouz A, Brocchi E, Reiche S, Guardado-Calvo P, Beer M, Rey FA Nat Commun. 2019 Feb 20;10(1):879. doi: 10.1038/s41467-019-08832-8. PMID:30787296^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Shi X, Kohl A, Leonard VH, Li P, McLees A, Elliott RM. Requirement of the N-terminal region of orthobunyavirus nonstructural protein NSm for virus assembly and morphogenesis. J Virol. 2006 Aug;80(16):8089-99. doi: 10.1128/JVI.00579-06. PMID:16873265 doi:http://dx.doi.org/10.1128/JVI.00579-06
↑ Hellert J, Aebischer A, Wernike K, Haouz A, Brocchi E, Reiche S, Guardado-Calvo P, Beer M, Rey FA. Orthobunyavirus spike architecture and recognition by neutralizing antibodies. Nat Commun. 2019 Feb 20;10(1):879. doi: 10.1038/s41467-019-08832-8. PMID:30787296 doi:http://dx.doi.org/10.1038/s41467-019-08832-8

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6h3v"

@@ Line 1: / Line 1: @@
 ==Bunyamwera Virus Glycoprotein Gc Head Domain==
-<StructureSection load='6h3v' size='340' side='right'  caption='[[6h3v]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
+<StructureSection load='6h3v' size='340' side='right'caption='[[6h3v]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6h3v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bunv Bunv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H3V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H3V FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6h3v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bunyamwera_virus Bunyamwera virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H3V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6H3V FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=35304 BUNV])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h3v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h3v OCA], [http://pdbe.org/6h3v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h3v RCSB], [http://www.ebi.ac.uk/pdbsum/6h3v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h3v ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6h3v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h3v OCA], [https://pdbe.org/6h3v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6h3v RCSB], [https://www.ebi.ac.uk/pdbsum/6h3v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6h3v ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/GP_BUNYW GP_BUNYW]] Glycoprotein N and Glycoprotein C interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion (By similarity).  Non-structural protein M: Plays a role virion budding at Golgi tubes and in subcellular location of Glycoprotein C protein.<ref>PMID:16873265</ref>
+[https://www.uniprot.org/uniprot/GP_BUNYW GP_BUNYW] Glycoprotein N and Glycoprotein C interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion (By similarity).  Non-structural protein M: Plays a role virion budding at Golgi tubes and in subcellular location of Glycoprotein C protein.<ref>PMID:16873265</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Bunv]]
+[[Category: Bunyamwera virus]]
-[[Category: Aebischer, A]]
+[[Category: Large Structures]]
-[[Category: Beer, M]]
+[[Category: Aebischer A]]
-[[Category: Brocchi, E]]
+[[Category: Beer M]]
-[[Category: Guardado-Calvo, P]]
+[[Category: Brocchi E]]
-[[Category: Haouz, A]]
+[[Category: Guardado-Calvo P]]
-[[Category: Hellert, J]]
+[[Category: Haouz A]]
-[[Category: Reiche, S]]
+[[Category: Hellert J]]
-[[Category: Rey, F A]]
+[[Category: Reiche S]]
-[[Category: Wernike, K]]
+[[Category: Rey FA]]
-[[Category: Envelope glycoprotein]]
+[[Category: Wernike K]]
-[[Category: Viral protein]]

6h3v

From Proteopedia

Current revision

Bunyamwera Virus Glycoprotein Gc Head Domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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