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| | ==Crystal structure of BauA, the Ferric preacinetobactin receptor from Acinetobacter baumannii== | | ==Crystal structure of BauA, the Ferric preacinetobactin receptor from Acinetobacter baumannii== |
| - | <StructureSection load='6h7v' size='340' side='right' caption='[[6h7v]], [[Resolution|resolution]] 2.54Å' scene=''> | + | <StructureSection load='6h7v' size='340' side='right'caption='[[6h7v]], [[Resolution|resolution]] 2.54Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6h7v]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Acib2 Acib2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H7V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H7V FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6h7v]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Acinetobacter_baumannii_ATCC_19606_=_CIP_70.34_=_JCM_6841 Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6H7V FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.54Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6h7f|6h7f]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6h7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h7v OCA], [https://pdbe.org/6h7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6h7v RCSB], [https://www.ebi.ac.uk/pdbsum/6h7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6h7v ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BIT33_06105, HMPREF0010_02301 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=575584 ACIB2])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h7v OCA], [http://pdbe.org/6h7v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h7v RCSB], [http://www.ebi.ac.uk/pdbsum/6h7v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h7v ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/D0CC21_ACIB2 D0CC21_ACIB2] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Acib2]] | + | [[Category: Acinetobacter baumannii ATCC 19606 = CIP 70 34 = JCM 6841]] |
| - | [[Category: Moynie, L]] | + | [[Category: Large Structures]] |
| - | [[Category: Naismith, J H]] | + | [[Category: Moynie L]] |
| - | [[Category: Baua]] | + | [[Category: Naismith JH]] |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Outer-membrane transporter]]
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| Structural highlights
Function
D0CC21_ACIB2
Publication Abstract from PubMed
New strategies are urgently required to develop antibiotics. The siderophore uptake system has attracted considerable attention, but rational design of siderophore antibiotic conjugates requires knowledge of recognition by the cognate outer-membrane transporter. Acinetobacter baumannii is a serious pathogen, which utilizes (pre)acinetobactin to scavenge iron from the host. We report the structure of the (pre)acinetobactin transporter BauA bound to the siderophore, identifying the structural determinants of recognition. Detailed biophysical analysis confirms that BauA recognises preacinetobactin. We show that acinetobactin is not recognised by the protein, thus preacinetobactin is essential for iron uptake. The structure shows and NMR confirms that under physiological conditions, a molecule of acinetobactin will bind to two free coordination sites on the iron preacinetobactin complex. The ability to recognise a heterotrimeric iron-preacinetobactin-acinetobactin complex may rationalize contradictory reports in the literature. These results open new avenues for the design of novel antibiotic conjugates (trojan horse) antibiotics.
Preacinetobactin not acinetobactin is essential for iron uptake by the BauA transporter of the pathogen Acinetobacter baumannii.,Moynie L, Serra I, Scorciapino MA, Oueis E, Page MG, Ceccarelli M, Naismith JH Elife. 2018 Dec 18;7. pii: 42270. doi: 10.7554/eLife.42270. PMID:30558715[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Moynie L, Serra I, Scorciapino MA, Oueis E, Page MG, Ceccarelli M, Naismith JH. Preacinetobactin not acinetobactin is essential for iron uptake by the BauA transporter of the pathogen Acinetobacter baumannii. Elife. 2018 Dec 18;7. pii: 42270. doi: 10.7554/eLife.42270. PMID:30558715 doi:http://dx.doi.org/10.7554/eLife.42270
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