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| | ==Crystal structure of Loei River virus GP1 glycoprotein at pH 5.0== | | ==Crystal structure of Loei River virus GP1 glycoprotein at pH 5.0== |
| - | <StructureSection load='6hj6' size='340' side='right' caption='[[6hj6]], [[Resolution|resolution]] 1.98Å' scene=''> | + | <StructureSection load='6hj6' size='340' side='right'caption='[[6hj6]], [[Resolution|resolution]] 1.98Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6hj6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Loei_river_mammarenavirus Loei river mammarenavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HJ6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HJ6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6hj6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mammarenavirus_loeiense Mammarenavirus loeiense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HJ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HJ6 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.98Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GPC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1437126 Loei River mammarenavirus])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hj6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hj6 OCA], [http://pdbe.org/6hj6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hj6 RCSB], [http://www.ebi.ac.uk/pdbsum/6hj6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hj6 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6hj6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hj6 OCA], [https://pdbe.org/6hj6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6hj6 RCSB], [https://www.ebi.ac.uk/pdbsum/6hj6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6hj6 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/A0A023J4Z7_9VIRU A0A023J4Z7_9VIRU]] Glycoprotein G1: interacts with the host receptor.[HAMAP-Rule:MF_04084] Glycoprotein G2: class I viral fusion protein that directs fusion of viral and host endosomal membranes, leading to delivery of the nucleocapsid into the cytoplasm. Membrane fusion is mediated by irreversible conformational changes induced upon acidification in the endosome.[HAMAP-Rule:MF_04084] Stable signal peptide (SSP): cleaved and functions as a signal peptide. In addition, it is also retained as the third component of the GP complex. The SSP is required for efficient glycoprotein expression, post-translational maturation cleavage of GP1 and GP2, glycoprotein transport to the cell surface plasma membrane, formation of infectious virus particles, and acid pH-dependent glycoprotein-mediated cell fusion.[HAMAP-Rule:MF_04084] | + | [https://www.uniprot.org/uniprot/A0A023J4Z7_9VIRU A0A023J4Z7_9VIRU] Glycoprotein G1: interacts with the host receptor.[HAMAP-Rule:MF_04084] Glycoprotein G2: class I viral fusion protein that directs fusion of viral and host endosomal membranes, leading to delivery of the nucleocapsid into the cytoplasm. Membrane fusion is mediated by irreversible conformational changes induced upon acidification in the endosome.[HAMAP-Rule:MF_04084] Stable signal peptide (SSP): cleaved and functions as a signal peptide. In addition, it is also retained as the third component of the GP complex. The SSP is required for efficient glycoprotein expression, post-translational maturation cleavage of GP1 and GP2, glycoprotein transport to the cell surface plasma membrane, formation of infectious virus particles, and acid pH-dependent glycoprotein-mediated cell fusion.[HAMAP-Rule:MF_04084] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 6hj6" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6hj6" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Glycoprotein GP 3D structures|Glycoprotein GP 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Loei river mammarenavirus]] | + | [[Category: Large Structures]] |
| - | [[Category: Bowden, T A]] | + | [[Category: Mammarenavirus loeiense]] |
| - | [[Category: Ng, W M]] | + | [[Category: Bowden TA]] |
| - | [[Category: Omari, K El]] | + | [[Category: El Omari K]] |
| - | [[Category: Pryce, R]] | + | [[Category: Ng WM]] |
| - | [[Category: Wagner, A]] | + | [[Category: Pryce R]] |
| - | [[Category: Watanabe, Y]] | + | [[Category: Wagner A]] |
| - | [[Category: Zeltina, A]] | + | [[Category: Watanabe Y]] |
| - | [[Category: Attachment]]
| + | [[Category: Zeltina A]] |
| - | [[Category: Glycoprotein]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| Structural highlights
Function
A0A023J4Z7_9VIRU Glycoprotein G1: interacts with the host receptor.[HAMAP-Rule:MF_04084] Glycoprotein G2: class I viral fusion protein that directs fusion of viral and host endosomal membranes, leading to delivery of the nucleocapsid into the cytoplasm. Membrane fusion is mediated by irreversible conformational changes induced upon acidification in the endosome.[HAMAP-Rule:MF_04084] Stable signal peptide (SSP): cleaved and functions as a signal peptide. In addition, it is also retained as the third component of the GP complex. The SSP is required for efficient glycoprotein expression, post-translational maturation cleavage of GP1 and GP2, glycoprotein transport to the cell surface plasma membrane, formation of infectious virus particles, and acid pH-dependent glycoprotein-mediated cell fusion.[HAMAP-Rule:MF_04084]
Publication Abstract from PubMed
The emergence of Old and New World arenaviruses from rodent reservoirs persistently threatens human health. The GP1 subunit of the envelope-displayed arenaviral glycoprotein spike complex, GPC, mediates host-cell recognition and is an important determinant of cross-species transmission. Previous structural analyses of Old World arenaviral GP1 glycoproteins, alone and in complex with a cognate GP2 subunit, have revealed that GP1 adopts two distinct conformational states, distinguished by differences in orientation of helical regions of the molecule. Here, through comparative study of the GP1 glycoprotein architectures of Old World Loei River virus and New World Whitewater Arroyo virus, we show that these rearrangements are restricted to Old World arenaviruses and are not solely induced by the pH change that is associated with virus endosomal trafficking. Our structure-based phylogenetic analysis of arenaviral GP1s provides a blueprint for understanding the discrete structural classes adopted by these therapeutically important targets.IMPORTANCE The genetically and geographically diverse group of viruses within the Arenaviridae family includes a number of zoonotic pathogens capable of causing fatal hemorrhagic fever. The multi-subunit GPC glycoprotein spike complex displayed on the arenavirus envelope is a key determinant of species tropism and the primary target of the host humoral immune response. Here, we show that the receptor-binding GP1 sub-component of the GPC spike from Old World but not New World arenaviruses adopts a distinct, pH-independent conformation in the absence of the cognate GP2. Our analysis provides a structure-based approach for understanding the discrete conformational classes sampled by these therapeutically important targets, informing strategies to develop arenaviral glycoprotein immunogens that resemble GPC, as presented on the mature virion surface.
Structure-based classification defines the discrete conformational classes adopted by the arenaviral GP1.,Pryce R, Ng WM, Zeltina A, Watanabe Y, El Omari K, Wagner A, Bowden TA J Virol. 2018 Oct 10. pii: JVI.01048-18. doi: 10.1128/JVI.01048-18. PMID:30305351[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Pryce R, Ng WM, Zeltina A, Watanabe Y, El Omari K, Wagner A, Bowden TA. Structure-based classification defines the discrete conformational classes adopted by the arenaviral GP1. J Virol. 2018 Oct 10. pii: JVI.01048-18. doi: 10.1128/JVI.01048-18. PMID:30305351 doi:http://dx.doi.org/10.1128/JVI.01048-18
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