6ztk
From Proteopedia
(Difference between revisions)
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<StructureSection load='6ztk' size='340' side='right'caption='[[6ztk]], [[Resolution|resolution]] 1.55Å' scene=''> | <StructureSection load='6ztk' size='340' side='right'caption='[[6ztk]], [[Resolution|resolution]] 1.55Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'> | + | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZTK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZTK FirstGlance]. <br> |
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55Å</td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRT:FRAGMENT+OF+TRITON+X-100'>TRT</scene></td></tr> | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRT:FRAGMENT+OF+TRITON+X-100'>TRT</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ztk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ztk OCA], [https://pdbe.org/6ztk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ztk RCSB], [https://www.ebi.ac.uk/pdbsum/6ztk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ztk ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ztk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ztk OCA], [https://pdbe.org/6ztk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ztk RCSB], [https://www.ebi.ac.uk/pdbsum/6ztk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ztk ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| - | == Function == | ||
| - | [https://www.uniprot.org/uniprot/V5H924_IXORI V5H924_IXORI] | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | The hard tick Ixodes ricinus is a vector of Lyme disease and tick-borne encephalitis. Host blood protein digestion, essential for tick development and reproduction, occurs in tick midgut digestive cells driven by cathepsin proteases. Little is known about the regulation of the digestive proteolytic machinery of I. ricinus. Here we characterize a novel cystatin-type protease inhibitor, mialostatin, from the I. ricinus midgut. Blood feeding rapidly induced mialostatin expression in the gut, which continued after tick detachment. Recombinant mialostatin inhibited a number of I. ricinus digestive cysteine cathepsins, with the greatest potency observed against cathepsin L isoforms, with which it co-localized in midgut digestive cells. The crystal structure of mialostatin was determined at 1.55 A to explain its unique inhibitory specificity. Finally, mialostatin effectively blocked in vitro proteolysis of blood proteins by midgut cysteine cathepsins. Mialostatin is likely to be involved in the regulation of gut-associated proteolytic pathways, making midgut cystatins promising targets for tick control strategies. | ||
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| - | Mialostatin, a Novel Midgut Cystatin from Ixodes ricinus Ticks: Crystal Structure and Regulation of Host Blood Digestion.,Kotal J, Busa M, Urbanova V, Rezacova P, Chmelar J, Langhansova H, Sojka D, Mares M, Kotsyfakis M Int J Mol Sci. 2021 May 20;22(10). pii: ijms22105371. doi: 10.3390/ijms22105371. PMID:34065290<ref>PMID:34065290</ref> | ||
| - | |||
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 6ztk" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: Ixodes ricinus]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Busa M]] | [[Category: Busa M]] | ||
[[Category: Mares M]] | [[Category: Mares M]] | ||
[[Category: Rezacova P]] | [[Category: Rezacova P]] | ||
Current revision
Crystal structure of Mialostatin, a gut cystatin from the hard tick Ixodes ricinus
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