1tto
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1tto.gif|left|200px]] | [[Image:1tto.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1tto", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | | | + | --> |
| - | | | + | {{STRUCTURE_1tto| PDB=1tto | SCENE= }} |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Crystal structure of the Rnase T1 variant R2''' | '''Crystal structure of the Rnase T1 variant R2''' | ||
| Line 24: | Line 21: | ||
Purine activity of RNase T1RV is further improved by substitution of Trp59 by tyrosine., Czaja R, Perbandt M, Betzel C, Hahn U, Biochem Biophys Res Commun. 2005 Oct 28;336(3):882-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16157302 16157302] | Purine activity of RNase T1RV is further improved by substitution of Trp59 by tyrosine., Czaja R, Perbandt M, Betzel C, Hahn U, Biochem Biophys Res Commun. 2005 Oct 28;336(3):882-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16157302 16157302] | ||
[[Category: Aspergillus oryzae]] | [[Category: Aspergillus oryzae]] | ||
| - | [[Category: Ribonuclease T(1)]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Betzel, C.]] | [[Category: Betzel, C.]] | ||
| Line 30: | Line 26: | ||
[[Category: Hahn, U.]] | [[Category: Hahn, U.]] | ||
[[Category: Perbandt, M.]] | [[Category: Perbandt, M.]] | ||
| - | [[Category: | + | [[Category: Adenine specificity]] |
| - | [[Category: | + | [[Category: Hydrolase]] |
| - | [[Category: | + | [[Category: Ribonuclease]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:21:03 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 07:21, 3 May 2008
Crystal structure of the Rnase T1 variant R2
Overview
Ribonuclease T1 is an enzyme that cleaves single-stranded RNA with high specificity after guanylyl residues. Although this enzyme is a very good characterized protein with respect to structure and enzymatic function, we were only recently successful in generating RNase T1-RV, a variant where the specificity was changed from guanine to purine. As this change of substrate specificity was made at the cost of activity, the aim was now to further improve the overall activity of the enzyme. Therefore, we have substituted the tryptophan in position 59 by tyrosine. This substitution led to an increase of enzymatic activity in comparison to variant RV to 425%. As the extent of this enhancement is unique so far we have crystallized and analyzed the structure of this variant in order to get more insights into the reasons for this. Here, we present the crystal structure of this so-called RNase T1-R2 at 2.1A resolution. The structure was determined by molecular replacement using the coordinates of the RV variant (PDB entry: 1Q9E). The data were refined to an R-factor of 18.7% and R(free) of 24%, respectively. The asymmetric unit contains three molecules and the crystal packing is very similar to that of variant RV.
About this Structure
1TTO is a Single protein structure of sequence from Aspergillus oryzae. Full crystallographic information is available from OCA.
Reference
Purine activity of RNase T1RV is further improved by substitution of Trp59 by tyrosine., Czaja R, Perbandt M, Betzel C, Hahn U, Biochem Biophys Res Commun. 2005 Oct 28;336(3):882-9. PMID:16157302 Page seeded by OCA on Sat May 3 10:21:03 2008
