8i2q
From Proteopedia
(Difference between revisions)
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8i2q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8i2q OCA], [https://pdbe.org/8i2q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8i2q RCSB], [https://www.ebi.ac.uk/pdbsum/8i2q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8i2q ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8i2q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8i2q OCA], [https://pdbe.org/8i2q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8i2q RCSB], [https://www.ebi.ac.uk/pdbsum/8i2q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8i2q ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/B2IF78_BEII9 B2IF78_BEII9] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The trisaccharide 1-kestose, a major constituent of fructooligosaccharide, has strong prebiotic effects. We used high-performance liquid chromatography and 1H nuclear magnetic resonance spectroscopy to show that BiBftA, a beta-fructosyltransferase belonging to glycoside hydrolase family 68, from Beijerinckia indica subsp. indica catalyzes transfructosylation of sucrose to produce mostly 1-kestose and levan polysaccharides. We substituted His395 and Phe473 in BiBftA with Arg and Tyr, respectively, and analyzed the reactions of the mutant enzymes with 180 g/L sucrose. The ratio of the molar concentrations of glucose and 1-kestose in the reaction mixture with wild-type BiBftA was 100:8.1, whereas that in the reaction mixture with the variant H395R/F473Y was 100:45.5, indicating that H395R/F473Y predominantly accumulated 1-kestose from sucrose. The X-ray crystal structure of H395R/F473Y suggests that its catalytic pocket is unfavorable for binding of sucrose while favorable for transfructosylation. | ||
| + | |||
| + | Characterization and alteration of product specificity of Beijerinckia indica subsp. indica beta-fructosyltransferase.,Li D, Miyasaka Y, Kubota A, Kozono T, Kitano Y, Sasaki N, Fujii T, Tochio T, Kadota Y, Nishikawa A, Tonozuka T Biosci Biotechnol Biochem. 2023 Aug 23;87(9):981-990. doi: 10.1093/bbb/zbad074. PMID:37280168<ref>PMID:37280168</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 8i2q" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Beijerinckia indica beta-fructosyltransferase variant H395R/F473Y
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