1tu4

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[[Image:1tu4.jpg|left|200px]]
[[Image:1tu4.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1tu4 |SIZE=350|CAPTION= <scene name='initialview01'>1tu4</scene>, resolution 2.20&Aring;
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The line below this paragraph, containing "STRUCTURE_1tu4", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=GDP:GUANOSINE-5&#39;-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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|ACTIVITY=
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|GENE= RAB5A, RAB5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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|DOMAIN=
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{{STRUCTURE_1tu4| PDB=1tu4 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tu4 OCA], [http://www.ebi.ac.uk/pdbsum/1tu4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1tu4 RCSB]</span>
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'''Crystal Structure of Rab5-GDP Complex'''
'''Crystal Structure of Rab5-GDP Complex'''
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[[Category: Zhang, X C.]]
[[Category: Zhang, X C.]]
[[Category: Zhu, G.]]
[[Category: Zhu, G.]]
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[[Category: gtpase]]
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[[Category: Gtpase]]
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[[Category: rab5]]
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[[Category: Rab5]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:22:00 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:01:10 2008''
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Revision as of 07:22, 3 May 2008

Image:1tu4.jpg

Template:STRUCTURE 1tu4

Crystal Structure of Rab5-GDP Complex


Overview

Rab5 is a small GTPase that regulates early endosome fusion. We present here the crystal structure of the Rab5 GTPase domain in complex with a GTP analog and the C-terminal domain of effector Rabaptin5. The proteins form a dyad-symmetric Rab5-Rabaptin5(2)-Rab5 ternary complex with a parallel coiled-coil Rabaptin5 homodimer in the middle. Two Rab5 molecules bind independently to the Rabaptin5 dimer using their switch and interswitch regions. The binding does not involve the Rab complementarity-determining regions. We also present the crystal structures of two distinct forms of GDP-Rab5 complexes, both of which are incompatible with Rabaptin5 binding. One has a dislocated and disordered switch I but a virtually intact switch II, whereas the other has its beta-sheet and both switch regions reorganized. Biochemical and functional analyses show that the crystallographically observed Rab5-Rabaptin5 complex also exists in solution, and disruption of this complex by mutation abrogates endosome fusion.

About this Structure

1TU4 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural basis of Rab5-Rabaptin5 interaction in endocytosis., Zhu G, Zhai P, Liu J, Terzyan S, Li G, Zhang XC, Nat Struct Mol Biol. 2004 Oct;11(10):975-83. Epub 2004 Sep 19. PMID:15378032 Page seeded by OCA on Sat May 3 10:22:00 2008

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