1tuk
From Proteopedia
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'''Crystal stucture of liganted type 2 non specific lipid transfer protein from wheat''' | '''Crystal stucture of liganted type 2 non specific lipid transfer protein from wheat''' | ||
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[[Category: Lamotte, F De.]] | [[Category: Lamotte, F De.]] | ||
[[Category: Pons, J L.]] | [[Category: Pons, J L.]] | ||
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| - | [[Category: | + | [[Category: Ns-ltp2]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:23:08 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 07:23, 3 May 2008
Crystal stucture of liganted type 2 non specific lipid transfer protein from wheat
Overview
In plants, a family of ubiquitous proteins named non-specific lipid-transfer proteins (ns-LTPs) facilitates the transfer of fatty acids, phospholipids and steroids between membranes. Recent data suggest that these secreted proteins play a key role in the formation of cuticular wax layers and in defence mechanisms against pathogens. In this study, X-ray crystallography has been used to examine the structural details of the interaction between a wheat type 2 ns-LTP and a lipid, L-alpha-palmitoyl-phosphatidyl glycerol. This crystal structure was solved ab initio at 1.12 A resolution by direct methods. The typical alpha-helical bundle fold of this protein is maintained by four disulfide bridges and delineates two hydrophobic cavities. The inner surface of the main cavity is lined by non-polar residues that provide a hydrophobic environment for the palmitoyl moiety of the lipid. The head-group region of this lipid protrudes from the surface and makes several polar interactions with a conserved patch of basic residues at the entrance of the pocket. The alkyl chain of a second lipid is bound within an adjacent smaller cavity. The structure shows that binding of the lipid tails to the protein involves extensive hydrophobic interactions.
About this Structure
1TUK is a Single protein structure of sequence from Triticum aestivum. Full crystallographic information is available from OCA.
Reference
Structure of a liganded type 2 non-specific lipid-transfer protein from wheat and the molecular basis of lipid binding., Hoh F, Pons JL, Gautier MF, de Lamotte F, Dumas C, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):397-406. Epub 2005, Mar 24. PMID:15805594 Page seeded by OCA on Sat May 3 10:23:08 2008
