8g4i
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==40S ribosomal subunit of the 80S Giardia intestinalis assemblage A ribosome with Emetine bound in V1 conformation== | |
| + | <StructureSection load='8g4i' size='340' side='right'caption='[[8g4i]], [[Resolution|resolution]] 3.24Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8g4i]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Giardia_intestinalis_assemblage_A Giardia intestinalis assemblage A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8G4I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8G4I FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.24Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=YAT:(2~{S},3~{R},11~{b}~{S})-2-[[(1~{R})-6,7-dimethoxy-1,2,3,4-tetrahydroisoquinolin-1-yl]methyl]-3-ethyl-9,10-dimethoxy-2,3,4,6,7,11~{b}-hexahydro-1~{H}-benzo[a]quinolizine'>YAT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8g4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8g4i OCA], [https://pdbe.org/8g4i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8g4i RCSB], [https://www.ebi.ac.uk/pdbsum/8g4i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8g4i ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/RSSA_GIAIC RSSA_GIAIC] Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits.[HAMAP-Rule:MF_03015] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Giardia lamblia is a deeply branching protist and a human pathogen. Its unusual biology presents the opportunity to explore conserved and fundamental molecular mechanisms. We determined the structure of the G. lamblia 80S ribosome bound to tRNA, mRNA, and the antibiotic emetine by cryo-electron microscopy, to an overall resolution of 2.49 A. The structure reveals rapidly evolving protein and nucleotide regions, differences in the peptide exit tunnel, and likely altered ribosome quality control pathways. Examination of translation initiation factor binding sites suggests these interactions are conserved despite a divergent initiation mechanism. Highlighting the potential of G. lamblia to resolve conserved biological principles; our structure reveals the interactions of the translation inhibitor emetine with the ribosome and mRNA, thus providing insight into the mechanism of action for this widely used antibiotic. Our work defines key questions in G. lamblia and motivates future experiments to explore the diversity of eukaryotic gene regulation. | ||
| - | + | The Giardia lamblia ribosome structure reveals divergence in several biological pathways and the mode of emetine function.,Eiler DR, Wimberly BT, Bilodeau DY, Taliaferro JM, Reigan P, Rissland OS, Kieft JS Structure. 2024 Apr 4;32(4):400-410.e4. doi: 10.1016/j.str.2023.12.015. Epub 2024 , Jan 18. PMID:38242118<ref>PMID:38242118</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Bilodeau | + | <div class="pdbe-citations 8g4i" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| + | [[Category: Giardia intestinalis assemblage A]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Bilodeau DY]] | ||
| + | [[Category: Eiler DR]] | ||
| + | [[Category: Kieft JS]] | ||
| + | [[Category: Rissland OS]] | ||
| + | [[Category: Wimberly BT]] | ||
Current revision
40S ribosomal subunit of the 80S Giardia intestinalis assemblage A ribosome with Emetine bound in V1 conformation
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