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1twx

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[[Image:1twx.gif|left|200px]]
[[Image:1twx.gif|left|200px]]
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{{Structure
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|PDB= 1twx |SIZE=350|CAPTION= <scene name='initialview01'>1twx</scene>, resolution 2.40&Aring;
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The line below this paragraph, containing "STRUCTURE_1twx", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=TYS:SULFONATED+TYROSINE'>TYS</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Thrombin Thrombin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.5 3.4.21.5] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= F2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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|DOMAIN=
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{{STRUCTURE_1twx| PDB=1twx | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1twx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1twx OCA], [http://www.ebi.ac.uk/pdbsum/1twx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1twx RCSB]</span>
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'''Crystal structure of the thrombin mutant D221A/D222K'''
'''Crystal structure of the thrombin mutant D221A/D222K'''
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[[Category: Tulinsky, A.]]
[[Category: Tulinsky, A.]]
[[Category: Zhang, E.]]
[[Category: Zhang, E.]]
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[[Category: thrombin]]
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[[Category: Thrombin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:28:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:02:12 2008''
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Revision as of 07:28, 3 May 2008

Image:1twx.gif

Template:STRUCTURE 1twx

Crystal structure of the thrombin mutant D221A/D222K


Contents

Overview

The thrombin mutant D221A/D222K (ARK) does not bind Na+ and has interesting functional properties intermediate between those of the slow and fast forms of wild type. We solved the X-ray crystal structure of ARK bound at exosite I with a fragment of hirudin at 2.4-A resolution. The structure shows a slight collapse of the 186 and 220 loops into the Na+ binding site due to disruption of the Asp222:Arg187 ion-pair. The backbone O atoms of Arg221a and Lys224 are shifted into conformations that eliminate optimal interaction with Na+. A paucity of solvent molecules in the Na+ binding site is also noted, by analogy to what is seen in the structure of the slow form. These findings reinforce the crucial role of the Asp222:Arg187 ion-pair in stabilizing the fast form of thrombin.

Disease

Known disease associated with this structure: Dysprothrombinemia OMIM:[176930], Hyperprothrombinemia OMIM:[176930], Hypoprothrombinemia OMIM:[176930]

About this Structure

1TWX is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of the thrombin mutant D221A/D222K: the Asp222:Arg187 ion-pair stabilizes the fast form., Pineda AO, Zhang E, Guinto ER, Savvides SN, Tulinsky A, Di Cera E, Biophys Chem. 2004 Dec 20;112(2-3):253-6. PMID:15572256 Page seeded by OCA on Sat May 3 10:28:16 2008

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