2hps

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of coelenterazine-binding protein from Renilla Muelleri

Structural highlights

2hps is a 1 chain structure with sequence from Renilla muelleri. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.72Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

C9V488_RENMU

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Bioluminescence in the sea pansy Renilla involves two distinct proteins, a Ca2+-triggered coelenterazine-binding protein (CBP), and Renilla luciferase. CBP contains one tightly bound coelenterazine molecule, which becomes available for reaction with luciferase and O2 only subsequent to Ca2+ binding. CBP belongs to the EF-hand superfamily of Ca2+-binding proteins and contains three "EF-hand" Ca2+-binding sites. The overall spatial structure of recombinant selenomethionine-labeled CBP determined at 1.7 A, is found to approximate the protein scaffold characteristic of the class of Ca2+-regulated photoproteins. Photoproteins however, catalyze molecular oxygen addition to coelenterazine producing a 2-hydroperoxycoelenterazine intermediate, which is stabilized within the binding cavity in the absence of Ca2+. Addition of Ca2+ triggers the bioluminescence reaction. However in CBP this first step of oxygen addition is not allowed. The different amino acid environments and hydrogen bond interactions within the binding cavity, are proposed to account for the different properties of the two classes of proteins.

Crystal structure of coelenterazine-binding protein from Renilla muelleri at 1.7 A: why it is not a calcium-regulated photoprotein.,Stepanyuk GA, Liu ZJ, Markova SS, Frank LA, Lee J, Vysotski ES, Wang BC Photochem Photobiol Sci. 2008 Apr;7(4):442-7. Epub 2008 Feb 19. PMID:18385886^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Stepanyuk GA, Liu ZJ, Markova SS, Frank LA, Lee J, Vysotski ES, Wang BC. Crystal structure of coelenterazine-binding protein from Renilla muelleri at 1.7 A: why it is not a calcium-regulated photoprotein. Photochem Photobiol Sci. 2008 Apr;7(4):442-7. Epub 2008 Feb 19. PMID:18385886 doi:10.1039/b716535h

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2hps"

@@ Line 3: / Line 3: @@
 <StructureSection load='2hps' size='340' side='right'caption='[[2hps]], [[Resolution|resolution]] 1.72&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2hps]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mueller's_sea_pansy Mueller's sea pansy]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HPS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HPS FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2hps]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Renilla_muelleri Renilla muelleri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HPS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HPS FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CTZ:C2-HYDROXY-COELENTERAZINE'>CTZ</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.72&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CTZ:C2-HYDROXY-COELENTERAZINE'>CTZ</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hps OCA], [https://pdbe.org/2hps PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hps RCSB], [https://www.ebi.ac.uk/pdbsum/2hps PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hps ProSAT], [https://www.topsan.org/Proteins/SECSG/2hps TOPSAN]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/C9V488_RENMU C9V488_RENMU]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 13: / Line 15: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hp/2hps_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 32: / Line 34: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Mueller's sea pansy]]
+[[Category: Renilla muelleri]]
-[[Category: Lee, J]]
+[[Category: Lee J]]
-[[Category: Liu, Z J]]
+[[Category: Liu ZJ]]
-[[Category: Rose, J P]]
+[[Category: Rose JP]]
-[[Category: Structural genomic]]
+[[Category: Stepanyuk G]]
-[[Category: Stepanyuk, G]]
+[[Category: Vysotski ES]]
-[[Category: Vysotski, E S]]
+[[Category: Wang BC]]
-[[Category: Wang, B C]]
-[[Category: Bioluminescence]]
-[[Category: Coelenterazine-binding]]
-[[Category: Luminescent protein]]
-[[Category: PSI, Protein structure initiative]]
-[[Category: Secsg]]

2hps

From Proteopedia

Current revision

Crystal structure of coelenterazine-binding protein from Renilla Muelleri

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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