2kqm

From Proteopedia

(Difference between revisions)

Current revision

Solution structure of the KI O18/O8 Y87H immunoglobulin light chain variable domain

Structural highlights

2kqm is a 2 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR, 20 models
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Light chain amyloidosis is a devastating protein misfolding disease characterized by the accumulation of amyloid fibrils that causes tissue damage and organ failure. These fibrils are composed of monoclonal light chain protein secreted from an abnormal proliferation of bone marrow plasma cells. We previously reported that amyloidogenic light chain protein AL-09 adopts an altered dimer while its germline protein (kappaI O18/O8) forms a canonical dimer observed in other light chain crystal structures. In solution, conformational heterogeneity obscures all NMR signals at the AL-09 and kappaI O18/O8 dimer interfaces, so we solved the nuclear magnetic resonance structure of two related mutants. AL-09 H87Y adopts the normal dimer interface, but the kappaI Y87H solution structure presents an altered interface rotated 180 degrees relative to the canonical dimer interface and 90 degrees from the AL-09 arrangement. Our results suggest that promiscuity in the light chain dimer interface may promote new intermolecular contacts that may contribute to amyloid fibril structure.

A single mutation promotes amyloidogenicity through a highly promiscuous dimer interface.,Peterson FC, Baden EM, Owen BA, Volkman BF, Ramirez-Alvarado M Structure. 2010 May 12;18(5):563-70. PMID:20462490^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Peterson FC, Baden EM, Owen BA, Volkman BF, Ramirez-Alvarado M. A single mutation promotes amyloidogenicity through a highly promiscuous dimer interface. Structure. 2010 May 12;18(5):563-70. PMID:20462490 doi:10.1016/j.str.2010.02.012

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2kqm"

@@ Line 1: / Line 1: @@
 ==Solution structure of the KI O18/O8 Y87H immunoglobulin light chain variable domain==
-<StructureSection load='2kqm' size='340' side='right'caption='[[2kqm]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2kqm' size='340' side='right'caption='[[2kqm]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2kqm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KQM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KQM FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2kqm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KQM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KQM FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2kqn|2kqn]]</div></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kqm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kqm OCA], [https://pdbe.org/2kqm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kqm RCSB], [https://www.ebi.ac.uk/pdbsum/2kqm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kqm ProSAT]</span></td></tr>
 </table>
@@ Line 12: / Line 12: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kq/2kqm_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 30: / Line 30: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Baden, E M]]
+[[Category: Baden EM]]
-[[Category: Peterson, F C]]
+[[Category: Peterson FC]]
-[[Category: Ramirez-Alvarado, M]]
+[[Category: Ramirez-Alvarado M]]
-[[Category: Volkman, B F]]
+[[Category: Volkman BF]]
-[[Category: Amyloidosis]]
-[[Category: Bence-jones protein]]
-[[Category: Disulfide bond]]
-[[Category: Homodimer]]
-[[Category: Immune system]]
-[[Category: Immunoglobulin domain]]
-[[Category: Immunoglobulin kappa light chain]]
-[[Category: Immunoglobulin v region]]

2kqm

From Proteopedia

Current revision

Solution structure of the KI O18/O8 Y87H immunoglobulin light chain variable domain

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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