3brj

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of mannitol operon repressor (MtlR) from Vibrio parahaemolyticus RIMD 2210633

Structural highlights

3brj is a 4 chain structure with sequence from Vibrio parahaemolyticus RIMD 2210633. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.75Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

Q87SQ4_VIBPA

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Many bacteria express phosphoenolpyruvate-dependent phosphotransferase systems (PTS). The mannitol-specific PTS catalyze the uptake and phosphorylation of d-mannitol. The uptake system comprises several genes encoded in the single operon. The expression of the mannitol operon is regulated by a proposed transcriptional factor, mannitol operon repressor (MtlR) that was first studied in Escherichia coli. Here we report the first crystal structures of MtlR from Vibrio parahemeolyticus (Vp-MtlR) and its homolog YggD protein from Shigella flexneri (Sf-YggD). MtlR and YggD belong to the same protein family (Pfam05068). Although Vp-MtlR and Sf-YggD share low sequence identity (22%), their overall structures are very similar, representing a novel all alpha-helical fold, and indicate similar function. However, their lack of any known DNA-binding structural motifs and their unfavorable electrostatic properties imply that MtlR/YggD are unlikely to bind a specific DNA operator directly as proposed earlier. This structural observation is further corroborated by in vitro DNA-binding studies of E. coli MtlR (Ec-MtlR), which detected no interaction of Ec-MtlR with the well characterized mannitol operator/promoter region. Therefore, MtlR/YggD belongs to a new class of transcription factors in bacteria that may regulate gene expression indirectly as a part of a larger transcriptional complex.

The mannitol operon repressor MtlR belongs to a new class of transcription regulators in bacteria.,Tan K, Clancy S, Borovilos M, Zhou M, Horer S, Moy S, Volkart LL, Sassoon J, Baumann U, Joachimiak A J Biol Chem. 2009 Dec 25;284(52):36670-9. Epub 2009 Oct 19. PMID:19840941^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Tan K, Clancy S, Borovilos M, Zhou M, Horer S, Moy S, Volkart LL, Sassoon J, Baumann U, Joachimiak A. The mannitol operon repressor MtlR belongs to a new class of transcription regulators in bacteria. J Biol Chem. 2009 Dec 25;284(52):36670-9. Epub 2009 Oct 19. PMID:19840941 doi:10.1074/jbc.M109.062679

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3brj"

@@ Line 3: / Line 3: @@
 <StructureSection load='3brj' size='340' side='right'caption='[[3brj]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3brj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibpa Vibpa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BRJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BRJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3brj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_parahaemolyticus_RIMD_2210633 Vibrio parahaemolyticus RIMD 2210633]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BRJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BRJ FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VP0368 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=223926 VIBPA])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3brj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3brj OCA], [https://pdbe.org/3brj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3brj RCSB], [https://www.ebi.ac.uk/pdbsum/3brj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3brj ProSAT], [https://www.topsan.org/Proteins/MCSG/3brj TOPSAN]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q87SQ4_VIBPA Q87SQ4_VIBPA]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 14: / Line 15: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/br/3brj_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 33: / Line 34: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Vibpa]]
+[[Category: Vibrio parahaemolyticus RIMD 2210633]]
-[[Category: Joachimiak, A]]
+[[Category: Joachimiak A]]
-[[Category: Structural genomic]]
+[[Category: Moy S]]
-[[Category: Moy, S]]
+[[Category: Tan K]]
-[[Category: Tan, K]]
+[[Category: Zhou M]]
-[[Category: Zhou, M]]
-[[Category: Apc85967 1]]
-[[Category: Mannitol operon repressor]]
-[[Category: Mcsg]]
-[[Category: Mtlr]]
-[[Category: PSI, Protein structure initiative]]
-[[Category: Transcription]]
-[[Category: Vibrio parahaemolyticus rimd 2210633]]

3brj

From Proteopedia

Current revision

Crystal structure of mannitol operon repressor (MtlR) from Vibrio parahaemolyticus RIMD 2210633

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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