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| | ==Structural basis for vesicle tethering by the Dsl1 complex== | | ==Structural basis for vesicle tethering by the Dsl1 complex== |
| - | <StructureSection load='3k8p' size='340' side='right' caption='[[3k8p]], [[Resolution|resolution]] 2.60Å' scene=''> | + | <StructureSection load='3k8p' size='340' side='right'caption='[[3k8p]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3k8p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824] and [http://en.wikipedia.org/wiki/Atcc_56498 Atcc 56498]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K8P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K8P FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3k8p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K8P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K8P FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KLLA0C02695g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28985 ATCC 56498]), SEC39, DSL3, YLR440C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k8p OCA], [http://pdbe.org/3k8p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3k8p RCSB], [http://www.ebi.ac.uk/pdbsum/3k8p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3k8p ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k8p OCA], [https://pdbe.org/3k8p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k8p RCSB], [https://www.ebi.ac.uk/pdbsum/3k8p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k8p ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SEC39_YEAST SEC39_YEAST]] Required for protein transport between the Golgi and the endoplasmic reticulum. May contribute to tethering of coatomer-coated retrograde transport vesicles to the ER membrane through interaction with and stabilization of the SNARE complex.<ref>PMID:15242642</ref> <ref>PMID:15958492</ref> | + | [https://www.uniprot.org/uniprot/Q6CUS2_KLULA Q6CUS2_KLULA] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | <jmolCheckbox> | | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k8/3k8p_consurf.spt"</scriptWhenChecked> | | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k8/3k8p_consurf.spt"</scriptWhenChecked> |
| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> | | </jmolCheckbox> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 18824]] | + | [[Category: Kluyveromyces lactis]] |
| - | [[Category: Atcc 56498]] | + | [[Category: Large Structures]] |
| - | [[Category: Hughson, F M]] | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Jeffrey, P D]] | + | [[Category: Hughson FM]] |
| - | [[Category: Ren, Y]] | + | [[Category: Jeffrey PD]] |
| - | [[Category: Dsl1 complex]] | + | [[Category: Ren Y]] |
| - | [[Category: Endoplasmic reticulum]]
| + | |
| - | [[Category: Er-golgi transport]]
| + | |
| - | [[Category: Intracellular trafficking]]
| + | |
| - | [[Category: Membrane]]
| + | |
| - | [[Category: Multisubunit tethering complex]]
| + | |
| - | [[Category: Protein transport]]
| + | |
| - | [[Category: Snare protein]]
| + | |
| - | [[Category: Transport]]
| + | |
| - | [[Category: Transport protein-transport protein complex]]
| + | |
| Structural highlights
Function
Q6CUS2_KLULA
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Vesicle trafficking requires membrane fusion, mediated by SNARE proteins, and upstream events that probably include "tethering," an initial long-range attachment between a vesicle and its target organelle. Among the factors proposed to mediate tethering are a set of multisubunit tethering complexes (MTCs). The Dsl1 complex, with only three subunits, is the simplest known MTC and is essential for the retrograde traffic of COPI-coated vesicles from the Golgi to the ER. To elucidate structural principles underlying MTC function, we have determined the structure of the Dsl1 complex, revealing a tower containing at its base the binding sites for two ER SNAREs and at its tip a flexible lasso for capturing vesicles. The Dsl1 complex binds to individual SNAREs via their N-terminal regulatory domains and also to assembled SNARE complexes; moreover, it is capable of accelerating SNARE complex assembly. Our results suggest that even the simplest MTC may be capable of orchestrating vesicle capture, uncoating, and fusion.
A structure-based mechanism for vesicle capture by the multisubunit tethering complex Dsl1.,Ren Y, Yip CK, Tripathi A, Huie D, Jeffrey PD, Walz T, Hughson FM Cell. 2009 Dec 11;139(6):1119-29. PMID:20005805[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ren Y, Yip CK, Tripathi A, Huie D, Jeffrey PD, Walz T, Hughson FM. A structure-based mechanism for vesicle capture by the multisubunit tethering complex Dsl1. Cell. 2009 Dec 11;139(6):1119-29. PMID:20005805 doi:10.1016/j.cell.2009.11.002
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