3s63

From Proteopedia

(Difference between revisions)

Current revision

Saposin-like protein Na-SLP-1

Structural highlights

3s63 is a 2 chain structure with sequence from Necator americanus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.7Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

H2L2M0_NECAM

Publication Abstract from PubMed

Saposin-like proteins (SAPLIPs) from soil-transmitted helminths play pivotal roles in host-pathogen interactions and have a high potential as targets for vaccination against parasitic diseases. We have identified two non-orthologous SAPLIPs from human and dog hookworm, Na-SLP-1 and Ac-SLP-1, and solved their three-dimensional crystal structures. Both proteins share the property of membrane binding as monitored by liposome co-pelleting assays and monolayer adsorption. Neither SAPLIP displayed any significant haemolytic or bactericidal activity. Based on the structural information, as well as the results from monolayer adsorption, we propose models of membrane interactions for both SAPLIPs. Initial membrane contact of the monomeric Na-SLP-1 is most likely by electrostatic interactions between the membrane surface and a prominent basic surface patch. In case of the dimeric Ac-SLP-1, membrane interactions are most likely initiated by a unique tryptophan residue that has previously been implicated in membrane interactions in other SAPLIPs.

Insights into the membrane interactions of the saposin-like proteins Na-SLP-1 and Ac-SLP-1 from human and dog hookworm.,Willis C, Wang CK, Osman A, Simon A, Pickering D, Mulvenna J, Riboldi-Tunicliffe A, Jones MK, Loukas A, Hofmann A PLoS One. 2011;6(10):e25369. Epub 2011 Oct 3. PMID:21991310^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Willis C, Wang CK, Osman A, Simon A, Pickering D, Mulvenna J, Riboldi-Tunicliffe A, Jones MK, Loukas A, Hofmann A. Insights into the membrane interactions of the saposin-like proteins Na-SLP-1 and Ac-SLP-1 from human and dog hookworm. PLoS One. 2011;6(10):e25369. Epub 2011 Oct 3. PMID:21991310 doi:10.1371/journal.pone.0025369

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3s63"

@@ Line 3: / Line 3: @@
 <StructureSection load='3s63' size='340' side='right'caption='[[3s63]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3s63]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_hookworm Human hookworm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S63 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S63 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3s63]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Necator_americanus Necator americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S63 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S63 FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3s64|3s64]]</div></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s63 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s63 OCA], [https://pdbe.org/3s63 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s63 RCSB], [https://www.ebi.ac.uk/pdbsum/3s63 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s63 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/H2L2M0_NECAM H2L2M0_NECAM]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Human hookworm]]
 [[Category: Large Structures]]
-[[Category: Hofmann, A]]
+[[Category: Necator americanus]]
-[[Category: Jones, M K]]
+[[Category: Hofmann A]]
-[[Category: Loukas, A]]
+[[Category: Jones MK]]
-[[Category: Mulvenna, J]]
+[[Category: Loukas A]]
-[[Category: Osman, A]]
+[[Category: Mulvenna J]]
-[[Category: Pickering, D]]
+[[Category: Osman A]]
-[[Category: Riboldi-Tunicliffe, A]]
+[[Category: Pickering D]]
-[[Category: Simon, A]]
+[[Category: Riboldi-Tunicliffe A]]
-[[Category: Wang, C K]]
+[[Category: Simon A]]
-[[Category: Willis, C]]
+[[Category: Wang CK]]
-[[Category: Lipid binding protein]]
+[[Category: Willis C]]
-[[Category: Lipid-binding]]
-[[Category: Saposin]]

3s63

From Proteopedia

Current revision

Saposin-like protein Na-SLP-1

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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