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| | <StructureSection load='3uez' size='340' side='right'caption='[[3uez]], [[Resolution|resolution]] 3.41Å' scene=''> | | <StructureSection load='3uez' size='340' side='right'caption='[[3uez]], [[Resolution|resolution]] 3.41Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3uez]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Ebvb9 Ebvb9] and [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UEZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UEZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3uez]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Human_herpesvirus_4_strain_B95-8 Human herpesvirus 4 strain B95-8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UEZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UEZ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.414Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ch8|2ch8]], [[1hmc|1hmc]], [[3uf2|3uf2]], [[3uf5|3uf5]]</div></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BARF1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10377 EBVB9]), CSF1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uez OCA], [https://pdbe.org/3uez PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uez RCSB], [https://www.ebi.ac.uk/pdbsum/3uez PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uez ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uez OCA], [https://pdbe.org/3uez PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uez RCSB], [https://www.ebi.ac.uk/pdbsum/3uez PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uez ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | == Disease == | |
| - | [[https://www.uniprot.org/uniprot/CSF1_HUMAN CSF1_HUMAN]] Note=Aberrant expression of CSF1 or CSF1R can promote cancer cell proliferation, invasion and formation of metastases. Overexpression of CSF1 or CSF1R is observed in a significant percentage of breast, ovarian, prostate, and endometrial cancers.<ref>PMID:16337366</ref> <ref>PMID:19934330</ref> Note=Aberrant expression of CSF1 or CSF1R may play a role in inflammatory diseases, such as rheumatoid arthritis, glomerulonephritis, atherosclerosis, and allograft rejection.<ref>PMID:16337366</ref> <ref>PMID:19934330</ref> | |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/BARF1_EBVB9 BARF1_EBVB9]] Plays diverse functions in immunomodulation and oncogenicity, maybe by acting as a functional receptor for human CSF1. May inhibit interferon secretion from mononuclear cells. Exhibits oncogenic activity in vitro.<ref>PMID:15064715</ref> <ref>PMID:15778977</ref> <ref>PMID:16054293</ref> [[https://www.uniprot.org/uniprot/CSF1_HUMAN CSF1_HUMAN]] Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.<ref>PMID:16337366</ref> <ref>PMID:19934330</ref> <ref>PMID:20829061</ref> <ref>PMID:20504948</ref>
| + | [https://www.uniprot.org/uniprot/BARF1_EBVB9 BARF1_EBVB9] Plays diverse functions in immunomodulation and oncogenicity, maybe by acting as a functional receptor for human CSF1. May inhibit interferon secretion from mononuclear cells. Exhibits oncogenic activity in vitro.<ref>PMID:15064715</ref> <ref>PMID:15778977</ref> <ref>PMID:16054293</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ebvb9]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Human]] | + | [[Category: Human herpesvirus 4 strain B95-8]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bracke, N]] | + | [[Category: Bracke N]] |
| - | [[Category: Elegheert, J]] | + | [[Category: Elegheert J]] |
| - | [[Category: Savvides, S N]] | + | [[Category: Savvides SN]] |
| - | [[Category: Cytokine]]
| + | |
| - | [[Category: Cytokine receptor-cytokine complex]]
| + | |
| - | [[Category: Cytokine/signaling]]
| + | |
| - | [[Category: Extracellular]]
| + | |
| - | [[Category: Four-helix bundle]]
| + | |
| - | [[Category: Glycoprotein]]
| + | |
| - | [[Category: Immunoglobulin domain]]
| + | |
| - | [[Category: Oncogene]]
| + | |
| - | [[Category: Protein complex]]
| + | |
| - | [[Category: Receptor]]
| + | |
| - | [[Category: Rtkiii]]
| + | |
| - | [[Category: Viral receptor]]
| + | |
| Structural highlights
Function
BARF1_EBVB9 Plays diverse functions in immunomodulation and oncogenicity, maybe by acting as a functional receptor for human CSF1. May inhibit interferon secretion from mononuclear cells. Exhibits oncogenic activity in vitro.[1] [2] [3]
Publication Abstract from PubMed
Hematopoietic human colony-stimulating factor 1 (hCSF-1) is essential for innate and adaptive immunity against viral and microbial infections and cancer. The human pathogen Epstein-Barr virus secretes the lytic-cycle protein BARF1 that neutralizes hCSF-1 to achieve immunomodulation. Here we show that BARF1 binds the dimer interface of hCSF-1 with picomolar affinity, away from the cognate receptor-binding site, to establish a long-lived complex featuring three hCSF-1 at the periphery of the BARF1 toroid. BARF1 locks dimeric hCSF-1 into an inactive conformation, rendering it unable to signal via its cognate receptor on human monocytes. This reveals a new functional role for hCSF-1 cooperativity in signaling. We propose a new viral strategy paradigm featuring an allosteric decoy receptor of the competitive type, which couples efficient sequestration and inactivation of the host growth factor to abrogate cooperative assembly of the cognate signaling complex.
Allosteric competitive inactivation of hematopoietic CSF-1 signaling by the viral decoy receptor BARF1.,Elegheert J, Bracke N, Pouliot P, Gutsche I, Shkumatov AV, Tarbouriech N, Verstraete K, Bekaert A, Burmeister WP, Svergun DI, Lambrecht BN, Vergauwen B, Savvides SN Nat Struct Mol Biol. 2012 Sep;19(9):938-47. doi: 10.1038/nsmb.2367. Epub 2012 Aug, 19. PMID:22902366[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Sall A, Caserta S, Jolicoeur P, Franqueville L, de Turenne-Tessier M, Ooka T. Mitogenic activity of Epstein-Barr virus-encoded BARF1 protein. Oncogene. 2004 Jun 17;23(28):4938-44. PMID:15064715 doi:10.1038/sj.onc.1207607
- ↑ Seto E, Yang L, Middeldorp J, Sheen TS, Chen JY, Fukayama M, Eizuru Y, Ooka T, Takada K. Epstein-Barr virus (EBV)-encoded BARF1 gene is expressed in nasopharyngeal carcinoma and EBV-associated gastric carcinoma tissues in the absence of lytic gene expression. J Med Virol. 2005 May;76(1):82-8. PMID:15778977 doi:10.1002/jmv.20327
- ↑ Wang Q, Tsao SW, Ooka T, Nicholls JM, Cheung HW, Fu S, Wong YC, Wang X. Anti-apoptotic role of BARF1 in gastric cancer cells. Cancer Lett. 2006 Jul 8;238(1):90-103. Epub 2005 Jul 27. PMID:16054293 doi:10.1016/j.canlet.2005.06.023
- ↑ Elegheert J, Bracke N, Pouliot P, Gutsche I, Shkumatov AV, Tarbouriech N, Verstraete K, Bekaert A, Burmeister WP, Svergun DI, Lambrecht BN, Vergauwen B, Savvides SN. Allosteric competitive inactivation of hematopoietic CSF-1 signaling by the viral decoy receptor BARF1. Nat Struct Mol Biol. 2012 Sep;19(9):938-47. doi: 10.1038/nsmb.2367. Epub 2012 Aug, 19. PMID:22902366 doi:10.1038/nsmb.2367
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