1tzy
From Proteopedia
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'''Crystal Structure of the Core-Histone Octamer to 1.90 Angstrom Resolution''' | '''Crystal Structure of the Core-Histone Octamer to 1.90 Angstrom Resolution''' | ||
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[[Category: Reynolds, C D.]] | [[Category: Reynolds, C D.]] | ||
[[Category: Wood, C M.]] | [[Category: Wood, C M.]] | ||
| - | [[Category: | + | [[Category: Histone-fold]] |
| - | [[Category: | + | [[Category: Tetramer-dimer-dimer]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:34:38 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 07:34, 3 May 2008
Crystal Structure of the Core-Histone Octamer to 1.90 Angstrom Resolution
Overview
Crystals of native histone octamers (H2A-H2B)-(H4-H3)-(H3'-H4')-(H2B'-H2A') from chick erythrocytes in 2 M KCl, 1.35 M potassium phosphate pH 6.9 diffract X-rays to 1.90 A resolution, yielding a structure with an R(work) value of 18.7% and an Rfree of 22.2%. The crystal space group is P6(5), the asymmetric unit of which contains one complete octamer. This high-resolution model of the histone-core octamer allows further insight into intermolecular interactions, including water molecules, that dock the histone dimers to the tetramer in the nucleosome-core particle and have relevance to nucleosome remodelling. The three key areas analysed are the H2A'-H3-H4 molecular cluster (also H2A-H3'-H4'), the H4-H2B' interaction (also H4'-H2B) and the H2A'-H4 beta-sheet interaction (also H2A-H4'). The latter of these three regions is important to nucleosome remodelling by RNA polymerase II, as it is shown to be a likely core-histone binding site, and its disruption creates an instability in the nucleosome-core particle. A majority of the water molecules in the high-resolution octamer have positions that correlate to similar positions in the high-resolution nucleosome-core particle structure, suggesting that the high-resolution octamer model can be used for comparative studies with the high-resolution nucleosome-core particle.
About this Structure
1TZY is a Protein complex structure of sequences from Gallus gallus. Full crystallographic information is available from OCA.
Reference
High-resolution structure of the native histone octamer., Wood CM, Nicholson JM, Lambert SJ, Chantalat L, Reynolds CD, Baldwin JP, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jun 1;61(Pt, 6):541-5. Epub 2005 Jun 1. PMID:16511091 Page seeded by OCA on Sat May 3 10:34:38 2008
