1u0b

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1u0b.gif|left|200px]]
[[Image:1u0b.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1u0b |SIZE=350|CAPTION= <scene name='initialview01'>1u0b</scene>, resolution 2.30&Aring;
+
The line below this paragraph, containing "STRUCTURE_1u0b", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cysteine--tRNA_ligase Cysteine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.16 6.1.1.16] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE= cysS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1u0b| PDB=1u0b | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u0b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0b OCA], [http://www.ebi.ac.uk/pdbsum/1u0b PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1u0b RCSB]</span>
+
-
}}
+
'''Crystal structure of cysteinyl-tRNA synthetase binary complex with tRNACys'''
'''Crystal structure of cysteinyl-tRNA synthetase binary complex with tRNACys'''
Line 30: Line 27:
[[Category: Perona, J J.]]
[[Category: Perona, J J.]]
[[Category: Zhang, C M.]]
[[Category: Zhang, C M.]]
-
[[Category: protein-rna complex]]
+
[[Category: Protein-rna complex]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:35:26 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:03:35 2008''
+

Revision as of 07:35, 3 May 2008

Image:1u0b.gif

Template:STRUCTURE 1u0b

Crystal structure of cysteinyl-tRNA synthetase binary complex with tRNACys


Overview

The crystal structure of Escherichia coli cysteinyl-tRNA synthetase (CysRS) bound to tRNA(Cys) at a resolution of 2.3 A reveals base-specific and shape-selective interactions across an extensive protein-RNA recognition interface. The complex contains a mixed alpha/beta C-terminal domain, which is disordered in the unliganded enzyme. This domain makes specific hydrogen bonding interactions with all three bases of the GCA anticodon. The tRNA anticodon stem is bent sharply toward the enzyme as compared with its conformation when bound to elongation factor Tu, providing an essential basis for shape-selective recognition. The CysRS structure also reveals interactions of conserved enzyme groups with the sugar-phosphate backbone in the D loop, adjacent to an unusual G15.G48 tertiary base pair previously implicated in tRNA aminoacylation. A combined mutational analysis of enzyme and tRNA groups at G15.G48 supports the notion that contacts between CysRS and the sugar-phosphate backbone contribute to recognition by indirect readout.

About this Structure

1U0B is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Shape-selective RNA recognition by cysteinyl-tRNA synthetase., Hauenstein S, Zhang CM, Hou YM, Perona JJ, Nat Struct Mol Biol. 2004 Nov;11(11):1134-41. Epub 2004 Oct 17. PMID:15489861 Page seeded by OCA on Sat May 3 10:35:26 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1u0b"
Personal tools