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| | ==Crystal structure of the anti-CRISPR protein, AcrF2== | | ==Crystal structure of the anti-CRISPR protein, AcrF2== |
| - | <StructureSection load='5yhr' size='340' side='right' caption='[[5yhr]], [[Resolution|resolution]] 1.34Å' scene=''> | + | <StructureSection load='5yhr' size='340' side='right'caption='[[5yhr]], [[Resolution|resolution]] 1.34Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5yhr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpd31 Bpd31]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YHR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YHR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5yhr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_virus_D3112 Pseudomonas virus D3112]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YHR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YHR FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.34Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">orf30 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10708 BPD31])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yhr OCA], [https://pdbe.org/5yhr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yhr RCSB], [https://www.ebi.ac.uk/pdbsum/5yhr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yhr ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yhr OCA], [http://pdbe.org/5yhr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yhr RCSB], [http://www.ebi.ac.uk/pdbsum/5yhr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yhr ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ACR30_BPD31 ACR30_BPD31]] Allows the phage to evade the CRISPR/Cas system type I-F.<ref>PMID:23242138</ref> | + | [https://www.uniprot.org/uniprot/ACR30_BPD31 ACR30_BPD31] Allows the phage to evade the CRISPR/Cas system type I-F.<ref>PMID:23242138</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bpd31]] | + | [[Category: Large Structures]] |
| - | [[Category: Bae, E]] | + | [[Category: Pseudomonas virus D3112]] |
| - | [[Category: Hong, S]] | + | [[Category: Bae E]] |
| - | [[Category: Ka, D]] | + | [[Category: Hong S]] |
| - | [[Category: Anti-crispr protein]] | + | [[Category: Ka D]] |
| - | [[Category: Viral protein]]
| + | |
| Structural highlights
Function
ACR30_BPD31 Allows the phage to evade the CRISPR/Cas system type I-F.[1]
Publication Abstract from PubMed
Clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) proteins provide microbial adaptive immunity against bacteriophages. In type I-F CRISPR-Cas systems, multiple Cas proteins (Csy1-4) compose a surveillance complex (Csy complex) with CRISPR RNA (crRNA) for target recognition. Here, we report the biochemical characterization of the Csy1-Csy2 subcomplex from Xanthomonas albilineans, including the analysis of its interaction with crRNA and AcrF2, an anti-CRISPR (Acr) protein from a phage that infects Pseudomonas aeruginosa The X. albilineans Csy1 and Csy2 proteins (XaCsy1 and XaCsy2, respectively) formed a stable heterodimeric complex that specifically bound the 8-nucleotide (nt) 5'-handle of the crRNA. In contrast, the XaCsy1-XaCsy2 heterodimer exhibited reduced affinity for the 28-nt X. albilineans CRISPR repeat RNA containing the 5'-handle sequence. Chromatographic and calorimetric analyses revealed tight binding between the Acr protein from the P. aeruginosa phage and the heterodimeric subunit of the X. albilineans Csy complex, suggesting that AcrF2 recognizes conserved features of Csy1-Csy2 heterodimers. We found that neither XaCsy1 nor XaCsy2 alone forms a stable complex with AcrF2 and the 5'-handle RNA, indicating that XaCsy1-XaCsy2 heterodimerization is required for binding them. We also solved the crystal structure of AcrF2 to a resolution of 1.34 A, enabling a more detailed structural analysis of the residues involved in the interactions with the Csy1-Csy2 heterodimer. Our results provide information about the order of events during the formation of the multisubunit crRNA-guided surveillance complex and suggest that the Acr protein inactivating type I-F CRISPR-Cas systems has broad specificity.
CRISPR RNA and anti-CRISPR protein binding to the Xanthomonas albilineans Csy1-Csy2 heterodimer in the type I-F CRISPR-Cas system.,Hong S, Ka D, Yoon SJ, Suh N, Jeong M, Suh JY, Bae E J Biol Chem. 2018 Feb 23;293(8):2744-2754. doi: 10.1074/jbc.RA117.001611. Epub, 2018 Jan 18. PMID:29348170[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR. Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system. Nature. 2013 Jan 17;493(7432):429-32. doi: 10.1038/nature11723. Epub 2012 Dec 16. PMID:23242138 doi:http://dx.doi.org/10.1038/nature11723
- ↑ Hong S, Ka D, Yoon SJ, Suh N, Jeong M, Suh JY, Bae E. CRISPR RNA and anti-CRISPR protein binding to the Xanthomonas albilineans Csy1-Csy2 heterodimer in the type I-F CRISPR-Cas system. J Biol Chem. 2018 Feb 23;293(8):2744-2754. doi: 10.1074/jbc.RA117.001611. Epub, 2018 Jan 18. PMID:29348170 doi:http://dx.doi.org/10.1074/jbc.RA117.001611
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