1u0u
From Proteopedia
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[[Image:1u0u.gif|left|200px]] | [[Image:1u0u.gif|left|200px]] | ||
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'''An Aldol Switch Discovered in Stilbene Synthases Mediates Cyclization Specificity of Type III Polyketide Synthases: Pine stilbene synthase structure''' | '''An Aldol Switch Discovered in Stilbene Synthases Mediates Cyclization Specificity of Type III Polyketide Synthases: Pine stilbene synthase structure''' | ||
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[[Category: Noel, J P.]] | [[Category: Noel, J P.]] | ||
[[Category: Schroder, J.]] | [[Category: Schroder, J.]] | ||
| - | [[Category: | + | [[Category: Aldol switch]] |
| - | [[Category: | + | [[Category: Alpha-beta-alpha-beta-alpha fold]] |
| - | [[Category: | + | [[Category: Catalytic triad]] |
| - | [[Category: | + | [[Category: Condensing enzyme]] |
| - | [[Category: | + | [[Category: Pk]] |
| - | [[Category: | + | [[Category: Thiolase fold]] |
| - | [[Category: | + | [[Category: Type iii polyketide synthase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:36:49 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 07:36, 3 May 2008
An Aldol Switch Discovered in Stilbene Synthases Mediates Cyclization Specificity of Type III Polyketide Synthases: Pine stilbene synthase structure
Overview
Stilbene synthase (STS) and chalcone synthase (CHS) each catalyze the formation of a tetraketide intermediate from a CoA-tethered phenylpropanoid starter and three molecules of malonyl-CoA, but use different cyclization mechanisms to produce distinct chemical scaffolds for a variety of plant natural products. Here we present the first STS crystal structure and identify, by mutagenic conversion of alfalfa CHS into a functional stilbene synthase, the structural basis for the evolution of STS cyclization specificity in type III polyketide synthase (PKS) enzymes. Additional mutagenesis and enzymatic characterization confirms that electronic effects rather than steric factors balance competing cyclization specificities in CHS and STS. Finally, we discuss the problematic in vitro reconstitution of plant stilbenecarboxylate pathways, using insights from existing biomimetic polyketide cyclization studies to generate a novel mechanistic hypothesis to explain stilbenecarboxylate biosynthesis.
About this Structure
1U0U is a Single protein structure of sequence from Pinus sylvestris. Full crystallographic information is available from OCA.
Reference
An aldol switch discovered in stilbene synthases mediates cyclization specificity of type III polyketide synthases., Austin MB, Bowman ME, Ferrer JL, Schroder J, Noel JP, Chem Biol. 2004 Sep;11(9):1179-94. PMID:15380179 Page seeded by OCA on Sat May 3 10:36:49 2008
