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7s8u
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[7s8u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S8U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S8U FirstGlance]. <br> | <table><tr><td colspan='2'>[[7s8u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S8U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S8U FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s8u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s8u OCA], [https://pdbe.org/7s8u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s8u RCSB], [https://www.ebi.ac.uk/pdbsum/7s8u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s8u ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.7Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s8u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s8u OCA], [https://pdbe.org/7s8u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s8u RCSB], [https://www.ebi.ac.uk/pdbsum/7s8u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s8u ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/F6SG69_HORSE F6SG69_HORSE] | |
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Mammalian peptide transporters, PepT1 and PepT2, mediate uptake of small peptides and are essential for their absorption. PepT also mediates absorption of many drugs and prodrugs to enhance their bioavailability. PepT has twelve transmembrane (TM) helices that fold into an N-terminal domain (NTD, TM1-6) and a C-terminal domain (CTD, TM7-12) and has a large extracellular domain (ECD) between TM9-10. It is well recognized that peptide transport requires movements of the NTD and CTD, but the role of the ECD in PepT1 remains unclear. Here we report the structure of horse PepT1 encircled in lipid nanodiscs and captured in the inward-open apo conformation. The structure shows that the ECD bridges the NTD and CTD by interacting with TM1. Deletion of ECD or mutations to the ECD-TM1 interface impairs the transport activity. These results demonstrate an important role of ECD in PepT1 and enhance our understanding of the transport mechanism in PepT1. | ||
| + | |||
| + | Extracellular domain of PepT1 interacts with TM1 to facilitate substrate transport.,Shen J, Hu M, Fan X, Ren Z, Portioli C, Yan X, Rong M, Zhou M Structure. 2022 Jul 7;30(7):1035-1041.e3. doi: 10.1016/j.str.2022.04.011. Epub , 2022 May 16. PMID:35580608<ref>PMID:35580608</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7s8u" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 11:39, 30 October 2024
Cryo-EM structure of a mammalian peptide transporter (PepT1/slc15a1) in nanodisc
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