8gar
From Proteopedia
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Cytochrome P460s are heme enzymes that oxidize hydroxylamine to nitrous oxide. They bear specialized "heme P460" cofactors that are cross-linked to their host polypeptides by a post-translationally modified lysine residue. Wild-type N. europaea cytochrome P460 may be isolated as a cross-link-deficient proenzyme following anaerobic overexpression in E. coli. When treated with peroxide, this proenzyme undergoes maturation to active enzyme with spectroscopic and catalytic properties that match wild-type cyt P460. This maturation reactivity requires no chaperones horizontal line it is intrinsic to the protein. This behavior extends to the broader cytochrome c'(beta) superfamily. Accumulated data reveal key contributions from the secondary coordination sphere that enable selective, complete maturation. Spectroscopic data support the intermediacy of a ferryl species along the maturation pathway. | Cytochrome P460s are heme enzymes that oxidize hydroxylamine to nitrous oxide. They bear specialized "heme P460" cofactors that are cross-linked to their host polypeptides by a post-translationally modified lysine residue. Wild-type N. europaea cytochrome P460 may be isolated as a cross-link-deficient proenzyme following anaerobic overexpression in E. coli. When treated with peroxide, this proenzyme undergoes maturation to active enzyme with spectroscopic and catalytic properties that match wild-type cyt P460. This maturation reactivity requires no chaperones horizontal line it is intrinsic to the protein. This behavior extends to the broader cytochrome c'(beta) superfamily. Accumulated data reveal key contributions from the secondary coordination sphere that enable selective, complete maturation. Spectroscopic data support the intermediacy of a ferryl species along the maturation pathway. | ||
| - | Cytochrome P460 Cofactor Maturation Proceeds via Peroxide-Dependent Post-translational Modification.,Bollmeyer MM, Coleman RE, Majer SH, Ferrao SD, Lancaster KM J Am Chem Soc. 2023 | + | Cytochrome P460 Cofactor Maturation Proceeds via Peroxide-Dependent Post-translational Modification.,Bollmeyer MM, Coleman RE, Majer SH, Ferrao SD, Lancaster KM J Am Chem Soc. 2023 Jul 5;145(26):14404-14416. doi: 10.1021/jacs.3c03608. Epub , 2023 Jun 20. PMID:37338957<ref>PMID:37338957</ref> |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
Current revision
Nitrosomonas europaea Cytochrome P460 Arg44Ala
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