8hgw
From Proteopedia
(Difference between revisions)
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<StructureSection load='8hgw' size='340' side='right'caption='[[8hgw]], [[Resolution|resolution]] 2.80Å' scene=''> | <StructureSection load='8hgw' size='340' side='right'caption='[[8hgw]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[8hgw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Gordonia | + | <table><tr><td colspan='2'>[[8hgw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Gordonia_sp._P8219 Gordonia sp. P8219]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HGW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HGW FirstGlance]. <br> |
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8000116Å</td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8000116Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1BO:1-BUTANOL'>1BO</scene>, <scene name='pdbligand=PHT:PHTHALIC+ACID'>PHT</scene></td></tr> | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1BO:1-BUTANOL'>1BO</scene>, <scene name='pdbligand=PHT:PHTHALIC+ACID'>PHT</scene></td></tr> | ||
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== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/Q2MHH5_9ACTN Q2MHH5_9ACTN] | [https://www.uniprot.org/uniprot/Q2MHH5_9ACTN Q2MHH5_9ACTN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Phthalate acid esters (PAEs), a group of xenobiotic compounds used extensively as plasticizers, have attracted increasing concern for adverse effects to human health and the environment. Microbial degradation relying on PAE hydrolases is a promising treatment. However, only a limited number of PAE hydrolases were characterized to date. Here we report the structures of MehpH, a monoalkyl phthalate (MBP) hydrolase that catalyzes the reaction of MBP to phthalic acid and the corresponding alcohol, in apo and ligand-bound form. The structures reveal a positively-charged catalytic center, complementary to the negatively-charged carboxyl group on MBP, and a penetrating tunnel that serves as exit of alcohol. The study provides a first glimpse into the enzyme-substrate binding model for PAE hydrolases, leading strong support to the development of better enzymes in the future. | ||
| + | |||
| + | Molecular insights into the catalytic mechanism of plasticizer degradation by a monoalkyl phthalate hydrolase.,Chen Y, Wang Y, Xu Y, Sun J, Yang L, Feng C, Wang J, Zhou Y, Zhang ZM, Wang Y Commun Chem. 2023 Mar 1;6(1):45. doi: 10.1038/s42004-023-00846-0. PMID:36859434<ref>PMID:36859434</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 8hgw" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: Gordonia]] | + | [[Category: Gordonia sp. P8219]] |
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Chen YB]] | [[Category: Chen YB]] | ||
[[Category: Wang YJ]] | [[Category: Wang YJ]] | ||
[[Category: Zhang ZM]] | [[Category: Zhang ZM]] | ||
Current revision
Crystal structure of MehpH in complex with MBP
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