1u25

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[[Image:1u25.gif|left|200px]]
[[Image:1u25.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1u25 |SIZE=350|CAPTION= <scene name='initialview01'>1u25</scene>, resolution 2.5&Aring;
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The line below this paragraph, containing "STRUCTURE_1u25", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=IHS:D-MYO-INOSITOL-HEXASULPHATE'>IHS</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/5-phytase 5-phytase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.72 3.1.3.72] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1u25| PDB=1u25 | SCENE= }}
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|RELATEDENTRY=[[1u24|1U24]], [[1u26|1U26]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u25 OCA], [http://www.ebi.ac.uk/pdbsum/1u25 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1u25 RCSB]</span>
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}}
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'''Crystal structure of Selenomonas ruminantium phytase complexed with persulfated phytate in the C2221 crystal form'''
'''Crystal structure of Selenomonas ruminantium phytase complexed with persulfated phytate in the C2221 crystal form'''
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[[Category: Tang, T Y.]]
[[Category: Tang, T Y.]]
[[Category: Wang, A H.J.]]
[[Category: Wang, A H.J.]]
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[[Category: p-loop]]
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[[Category: P-loop]]
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[[Category: phytase]]
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[[Category: Phytase]]
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[[Category: ptp]]
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[[Category: Ptp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:39:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:04:24 2008''
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Revision as of 07:39, 3 May 2008

Image:1u25.gif

Template:STRUCTURE 1u25

Crystal structure of Selenomonas ruminantium phytase complexed with persulfated phytate in the C2221 crystal form


Overview

Various inositide phosphatases participate in the regulation of inositol polyphosphate signaling molecules. Plant phytases are phosphatases that hydrolyze phytate to less-phosphorylated myo-inositol derivatives and phosphate. The phytase from Selenomonas ruminantium shares no sequence homology with other microbial phytases. Its crystal structure revealed a phytase fold of the dual-specificity phosphatase type. The active site is located near a conserved cysteine-containing (Cys241) P loop. We also solved two other crystal forms in which an inhibitor, myo-inositol hexasulfate, is cocrystallized with the enzyme. In the "standby" and the "inhibited" crystal forms, the inhibitor is bound, respectively, in a pocket slightly away from Cys241 and at the substrate binding site where the phosphate group to be hydrolyzed is held close to the -SH group of Cys241. Our structural and mutagenesis studies allow us to visualize the way in which the P loop-containing phytase attracts and hydrolyzes the substrate (phytate) sequentially.

About this Structure

1U25 is a Single protein structure of sequence from Selenomonas ruminantium. Full crystallographic information is available from OCA.

Reference

Structures of Selenomonas ruminantium phytase in complex with persulfated phytate: DSP phytase fold and mechanism for sequential substrate hydrolysis., Chu HM, Guo RT, Lin TW, Chou CC, Shr HL, Lai HL, Tang TY, Cheng KJ, Selinger BL, Wang AH, Structure. 2004 Nov;12(11):2015-24. PMID:15530366 Page seeded by OCA on Sat May 3 10:39:44 2008

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