1u2k
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1u2k.jpg|left|200px]] | [[Image:1u2k.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1u2k", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_1u2k| PDB=1u2k | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Crystal structure of the C-terminal domain from the catalase-peroxidase KatG of Escherichia coli (I41)''' | '''Crystal structure of the C-terminal domain from the catalase-peroxidase KatG of Escherichia coli (I41)''' | ||
| Line 30: | Line 27: | ||
[[Category: Loewen, P C.]] | [[Category: Loewen, P C.]] | ||
[[Category: Melik-Adamyan, W.]] | [[Category: Melik-Adamyan, W.]] | ||
| - | [[Category: | + | [[Category: C-terminal domain]] |
| - | [[Category: | + | [[Category: Catalase-peroxidase]] |
| - | [[Category: | + | [[Category: Katg]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:40:44 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 07:40, 3 May 2008
Crystal structure of the C-terminal domain from the catalase-peroxidase KatG of Escherichia coli (I41)
Overview
Catalase-peroxidases or KatGs, the apparent in vivo activators of the anti-tubercular pro-drug isoniazid, are active as homodimers, each subunit having two distinct but sequence- and structure-related domains. The N-terminal domain contains the haem group and is catalytically active, while the C-terminal domain lacks the cofactor. The C-terminal domain of KatG from Escherichia coli is expressed as a soluble protein which has been crystallized in triclinic, orthorhombic and tetragonal crystal forms. Packing in the orthorhombic crystals, with eight molecules in the asymmetric unit, follows the pattern of commensurate modulated structures, which explains the diversity of pseudo-origin peaks observed in the native Patterson map. The different crystal forms arise from variations in the length and sequence of the N-terminal extensions in the different constructs. Despite the variability in the N-terminal region, the overall domain conformations beginning with Pro437 are very similar both to each other and to the C-terminal domains within the native structures of the KatGs from Haloarcula marismortui and Burkholderia pseudomallei. Some structural reorganization in the C-terminal domain relative to the N-terminal domain has evolved to compensate for the absence of the haem group. A high percentage of the residues in the C-terminal domains of KatG proteins from different sources are highly conserved and these residues are spread uniformly throughout the domain. The easily folded nature and retention of structure in the C-terminal domain suggests that it may serve as a platform for the folding of the N-terminal domain and for stabilization of the molecular dimer.
About this Structure
1U2K is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structure of the C-terminal domain of the catalase-peroxidase KatG from Escherichia coli., Carpena X, Melik-Adamyan W, Loewen PC, Fita I, Acta Crystallogr D Biol Crystallogr. 2004 Oct;60(Pt 10):1824-32. Epub 2004, Sep 23. PMID:15388929 Page seeded by OCA on Sat May 3 10:40:44 2008
