8r3w
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of a homospecific CR57 diabody== | |
| + | <StructureSection load='8r3w' size='340' side='right'caption='[[8r3w]], [[Resolution|resolution]] 2.38Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8r3w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8R3W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8R3W FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.38Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8r3w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8r3w OCA], [https://pdbe.org/8r3w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8r3w RCSB], [https://www.ebi.ac.uk/pdbsum/8r3w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8r3w ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Host-cell entry of the highly pathogenic rabies virus (RABV) is mediated by glycoprotein (G) spikes, which also comprise the primary target for the humoral immune response. RABV glycoprotein (RABV-G) displays several antigenic sites that are targeted by neutralizing monoclonal antibodies (mAbs). In this study, we determined the epitope of a potently neutralizing human mAb, CR57, which we engineered into a diabody format to facilitate crystallization. We report the crystal structure of the CR57 diabody alone at 2.38 A resolution, and in complex with RABV-G domain III at 2.70 A resolution. The CR57-RABV-G structure reveals critical interactions at the antigen interface, which target the conserved "KLCGVL" peptide and residues proximal to it on RABV-G. Structural analysis combined with a cell-cell fusion assay demonstrates that CR57 effectively inhibits RABV-G-mediated fusion by obstructing the fusogenic transitions of the spike protein. Altogether, this investigation provides a structural perspective on RABV inhibition by a potently neutralizing human antibody. | ||
| - | + | Structural insight into rabies virus neutralization revealed by an engineered antibody scaffold.,Kedari A, Iheozor-Ejiofor R, Salminen P, Ugurlu H, Makela AR, Levanov L, Vapalahti O, Hytonen VP, Saksela K, Rissanen I Structure. 2024 Oct 25:S0969-2126(24)00437-4. doi: 10.1016/j.str.2024.10.002. PMID:39471803<ref>PMID:39471803</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 8r3w" style="background-color:#fffaf0;"></div> |
| - | [[Category: Kedari | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Kedari A]] | ||
| + | [[Category: Rissanen I]] | ||
Current revision
Crystal structure of a homospecific CR57 diabody
| |||||||||||
