1u4l

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[[Image:1u4l.gif|left|200px]]
[[Image:1u4l.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1u4l |SIZE=350|CAPTION= <scene name='initialview01'>1u4l</scene>, resolution 2.00&Aring;
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The line below this paragraph, containing "STRUCTURE_1u4l", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=H1S:HEPARIN+DISACCHARIDE+I-S'>H1S</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= CCL5, SCYA5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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-->
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|DOMAIN=
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{{STRUCTURE_1u4l| PDB=1u4l | SCENE= }}
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|RELATEDENTRY=[[1hrj|1HRJ]], [[1rtn|1RTN]], [[1rto|1RTO]], [[1b3a|1B3A]], [[1eqt|1EQT]], [[1u4r|1U4R]], [[1u4m|1U4M]], [[1u4p|1U4P]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u4l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u4l OCA], [http://www.ebi.ac.uk/pdbsum/1u4l PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1u4l RCSB]</span>
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}}
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'''human RANTES complexed to heparin-derived disaccharide I-S'''
'''human RANTES complexed to heparin-derived disaccharide I-S'''
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[[Category: Wells, T N.C.]]
[[Category: Wells, T N.C.]]
[[Category: Zwahlen, C.]]
[[Category: Zwahlen, C.]]
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[[Category: chemoattractant]]
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[[Category: Chemoattractant]]
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[[Category: chemokine]]
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[[Category: Chemokine]]
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[[Category: cytokine]]
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[[Category: Cytokine]]
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[[Category: glycosaminoglycan]]
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[[Category: Glycosaminoglycan]]
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[[Category: protein-heparin disaccharide complex]]
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[[Category: Protein-heparin disaccharide complex]]
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[[Category: rante]]
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[[Category: Rante]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:45:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:05:22 2008''
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Revision as of 07:45, 3 May 2008

Image:1u4l.gif

Template:STRUCTURE 1u4l

human RANTES complexed to heparin-derived disaccharide I-S


Overview

The biological activity of chemokines requires interactions with cell surface proteoglycans. We have determined the structure of the chemokine RANTES (regulated on activation normal T cell expressed) in the presence of heparin-derived disaccharide analogs by X-ray crystallography. These structures confirm the essential role of the BBXB motif in the interaction between the chemokine and the disaccharide. Unexpected interactions were observed in the 30s loop and at the amino terminus. Mutant RANTES molecules were designed to abrogate these interactions and their biological activity examined in vivo. The K45E mutant within the BBXB motif lost the capacity to bind heparin and the ability to elicit cellular recruitment. The Y3A mutant maintained its capacity to bind heparin but was unable to elicit cellular recruitment. Finally, a tetrasaccharide is the smallest oligosaccharide which effectively abolishes the ability of RANTES to recruit cells in vivo. These crystallographic structures provide a description of the molecular interaction of a chemokine with glycosaminoglycans.

About this Structure

1U4L is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The X-ray structure of RANTES: heparin-derived disaccharides allows the rational design of chemokine inhibitors., Shaw JP, Johnson Z, Borlat F, Zwahlen C, Kungl A, Roulin K, Harrenga A, Wells TN, Proudfoot AE, Structure. 2004 Nov;12(11):2081-93. PMID:15530372 Page seeded by OCA on Sat May 3 10:45:20 2008

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OCA

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