3bws

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of the leptospiral antigen Lp49

Structural highlights

3bws is a 2 chain structure with sequence from Leptospira interrogans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.99Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LP49_LEPIC May be involved in virulence. Binds human plasminogen (PLG) and stimulates its proteolytic cleavage to enzymatically active plasmin in the presence of an urokinase-type PLG activator in vitro. Activated plasmin has proteolytic activity which may help the bacteria to spread throughout the host by degrading extracellular matrix components, facilitating tissue penetration and invasion.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Pathogenic Leptospira is the etiological agent of leptospirosis, a life-threatening disease that affects populations worldwide. Currently available vaccines have limited effectiveness and therapeutic interventions are complicated by the difficulty in making an early diagnosis of leptospirosis. The genome of Leptospira interrogans was recently sequenced and comparative genomic analysis contributed to the identification of surface antigens, potential candidates for development of new vaccines and serodiagnosis. Lp49 is a membrane-associated protein recognized by antibodies present in sera from early and convalescent phases of leptospirosis patients. Its crystal structure was determined by single-wavelength anomalous diffraction using selenomethionine-labelled crystals and refined at 2.0 A resolution. Lp49 is composed of two domains and belongs to the all-beta-proteins class. The N-terminal domain folds in an immunoglobulin-like beta-sandwich structure, whereas the C-terminal domain presents a seven-bladed beta-propeller fold. Structural analysis of Lp49 indicates putative protein-protein binding sites, suggesting a role in Leptospira-host interaction. This is the first crystal structure of a leptospiral antigen described to date.

The leptospiral antigen Lp49 is a two-domain protein with putative protein binding function.,Giuseppe PO, Neves FO, Nascimento AL, Guimaraes BG J Struct Biol. 2008 Jul;163(1):53-60. Epub 2008 Apr 14. PMID:18508281^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Vieira ML, Atzingen MV, Oliveira TR, Oliveira R, Andrade DM, Vasconcellos SA, Nascimento AL. In vitro identification of novel plasminogen-binding receptors of the pathogen Leptospira interrogans. PLoS One. 2010 Jun 22;5(6):e11259. PMID:20582320 doi:10.1371/journal.pone.0011259
↑ Giuseppe PO, Neves FO, Nascimento AL, Guimaraes BG. The leptospiral antigen Lp49 is a two-domain protein with putative protein binding function. J Struct Biol. 2008 Jul;163(1):53-60. Epub 2008 Apr 14. PMID:18508281 doi:10.1016/j.jsb.2008.04.003

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3bws"

@@ Line 3: / Line 3: @@
 <StructureSection load='3bws' size='340' side='right'caption='[[3bws]], [[Resolution|resolution]] 1.99&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3bws]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"leptospira_icteroides"_noguchi_1919 "leptospira icteroides" noguchi 1919]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BWS FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3bws]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leptospira_interrogans Leptospira interrogans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BWS FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.99&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LIC10793 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=173 "Leptospira icteroides" Noguchi 1919])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bws OCA], [https://pdbe.org/3bws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bws RCSB], [https://www.ebi.ac.uk/pdbsum/3bws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bws ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/LP49_LEPIC LP49_LEPIC] May be involved in virulence. Binds human plasminogen (PLG) and stimulates its proteolytic cleavage to enzymatically active plasmin in the presence of an urokinase-type PLG activator in vitro. Activated plasmin has proteolytic activity which may help the bacteria to spread throughout the host by degrading extracellular matrix components, facilitating tissue penetration and invasion.<ref>PMID:20582320</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 14: / Line 15: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bw/3bws_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 32: / Line 33: @@
 __TOC__
 </StructureSection>
-[[Category: Leptospira icteroides noguchi 1919]]
 [[Category: Large Structures]]
-[[Category: Giuseppe, P O]]
+[[Category: Leptospira interrogans]]
-[[Category: Guimaraes, B G]]
+[[Category: Giuseppe PO]]
-[[Category: Nascimento, A L.T O]]
+[[Category: Guimaraes BG]]
-[[Category: Neves, F O]]
+[[Category: Nascimento ALTO]]
-[[Category: 7-bladed beta propeller]]
+[[Category: Neves FO]]
-[[Category: Immunoglobulin-like]]
-[[Category: Two-domain]]
-[[Category: Unknown function]]

3bws

From Proteopedia

Current revision

Crystal structure of the leptospiral antigen Lp49

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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