3l48

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of the C-terminal domain of the PapC usher

Structural highlights

3l48 is a 5 chain structure with sequence from Escherichia coli UTI89. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.1Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q1R2W8_ECOUT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

P pili are extracellular appendages responsible for the targeting of uropathogenic Escherichia coli to the kidney. They are assembled by the chaperone-usher (CU) pathway of pilus biogenesis involving two proteins, the periplasmic chaperone PapD and the outer membrane assembly platform, PapC. Many aspects of the structural biology of the Pap CU pathway have been elucidated, except for the C-terminal domain of the PapC usher, the structure of which is unknown. In this report, we identify a stable and folded fragment of the C-terminal region of the PapC usher and determine its structure using both X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy. These structures reveal a beta-sandwich fold very similar to that of the plug domain, a domain of PapC obstructing its translocation domain. This structural similarity suggests similar functions in usher-mediated pilus biogenesis, playing out at different stages of the process. This structure paves the way for further functional analysis targeting surfaces common to both the plug and the C-terminal domain of PapC.

Structural homology between the C-terminal domain of the PapC usher and its plug.,Ford B, Rego AT, Ragan TJ, Pinkner J, Dodson K, Driscoll PC, Hultgren S, Waksman G J Bacteriol. 2010 Apr;192(7):1824-31. Epub 2010 Jan 29. PMID:20118254^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ford B, Rego AT, Ragan TJ, Pinkner J, Dodson K, Driscoll PC, Hultgren S, Waksman G. Structural homology between the C-terminal domain of the PapC usher and its plug. J Bacteriol. 2010 Apr;192(7):1824-31. Epub 2010 Jan 29. PMID:20118254 doi:10.1128/JB.01677-09

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3l48"

Categories: Escherichia coli UTI89 | Large Structures | Ford BA | Hultgren SJ

@@ Line 1: / Line 1: @@
 ==Crystal structure of the C-terminal domain of the PapC usher==
-<StructureSection load='3l48' size='340' side='right' caption='[[3l48]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
+<StructureSection load='3l48' size='340' side='right'caption='[[3l48]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3l48]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecout Ecout]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L48 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3L48 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3l48]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_UTI89 Escherichia coli UTI89]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L48 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3L48 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">papC, UTI89_C4893 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=364106 ECOUT])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3l48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l48 OCA], [http://pdbe.org/3l48 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3l48 RCSB], [http://www.ebi.ac.uk/pdbsum/3l48 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3l48 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3l48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l48 OCA], [https://pdbe.org/3l48 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3l48 RCSB], [https://www.ebi.ac.uk/pdbsum/3l48 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3l48 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q1R2W8_ECOUT Q1R2W8_ECOUT]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 13: / Line 15: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l4/3l48_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 27: / Line 29: @@
 </div>
 <div class="pdbe-citations 3l48" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Adhesin 3D structures|Adhesin 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Ecout]]
+[[Category: Escherichia coli UTI89]]
-[[Category: Ford, B A]]
+[[Category: Large Structures]]
-[[Category: Hultgren, S J]]
+[[Category: Ford BA]]
-[[Category: Cell outer membrane]]
+[[Category: Hultgren SJ]]
-[[Category: Fimbrium]]
-[[Category: Greek key]]
-[[Category: Ig fold]]
-[[Category: Membrane]]
-[[Category: Transmembrane]]
-[[Category: Transport]]
-[[Category: Transport protein]]

3l48

From Proteopedia

Current revision

Crystal structure of the C-terminal domain of the PapC usher

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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