3tm6

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== Function ==
== Function ==
[https://www.uniprot.org/uniprot/B2MG_HUMAN B2MG_HUMAN] Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
[https://www.uniprot.org/uniprot/B2MG_HUMAN B2MG_HUMAN] Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
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== Publication Abstract from PubMed ==
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beta-2 microglobulin (beta2m) is an amyloidogenic protein responsible for dialysis-related amyloidosis in man. In the early stages of amyloid fibril formation, beta2m associates into dimers and higher oligomers, although the structural details of such aggregates are poorly understood. To characterize the protein-protein interactions supporting the formation of oligomers, three individual beta2m cysteine mutants and their disulfide-linked homodimers (DIMC20, DIMC50 and DIMC60) were prepared. Amyloid propensity, oligomerization state in solution and crystallogenesis were tested for each beta2m homodimer: DIMC20 and DIMC50 display a mixture of tetrameric and dimeric species in solution and also yield protein crystals and amyloid fibrils, whereas DIMC60 is dimeric in solution but does not form protein crystals nor amyloid fibrils. The X-ray structures of DIMC20 and DIMC50 show that the two engineered dimers form a tetrameric assembly; for both tetrameric species, the noncovalent association interface is based on the interaction of facing beta2m D-strands and is conserved. Notably, DIMC20 and DIMC50 trigger amyloid formation in wild-type beta2m in unseeded reactions. Thus, when the D-D-strand interface is impaired by an intermolecular disulfide bond (as in DIMC60), the formation of tetramers is hindered, and the protein is not amyloidogenic and does not promote amyloid aggregation of wild-type beta2m. Implications for beta2m oligomerization are discussed.
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A recurrent D-strand association interface is observed in beta-2 microglobulin oligomers.,Colombo M, de Rosa M, Bellotti V, Ricagno S, Bolognesi M FEBS J. 2012 Mar;279(6):1131-43. doi: 10.1111/j.1742-4658.2012.08510.x. Epub 2012, Feb 23. PMID:22289140<ref>PMID:22289140</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
==See Also==

Current revision

Crystal structure of the beta-2 microglobulin DIMC50 disulphide-linked homodimer mutant

PDB ID 3tm6

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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