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| | <StructureSection load='3zlc' size='340' side='right'caption='[[3zlc]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='3zlc' size='340' side='right'caption='[[3zlc]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3zlc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZLC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZLC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3zlc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZLC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZLC FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zlc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zlc OCA], [https://pdbe.org/3zlc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zlc RCSB], [https://www.ebi.ac.uk/pdbsum/3zlc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zlc ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.999Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zlc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zlc OCA], [https://pdbe.org/3zlc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zlc RCSB], [https://www.ebi.ac.uk/pdbsum/3zlc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zlc ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/ERV41_YEAST ERV41_YEAST]] Constituent of COPII-coated endoplasmic reticulum-derived transport vesicles. Required for efficient transport of a subset of secretory proteins to the Golgi. The C-terminal Ile-Leu motif is required for exit from the endoplasmic reticulum. Facilitates retrograde transport from the Golgi to the endoplasmic reticulum.<ref>PMID:11157978</ref> <ref>PMID:12426381</ref>
| + | [https://www.uniprot.org/uniprot/ERV41_YEAST ERV41_YEAST] Constituent of COPII-coated endoplasmic reticulum-derived transport vesicles. Required for efficient transport of a subset of secretory proteins to the Golgi. The C-terminal Ile-Leu motif is required for exit from the endoplasmic reticulum. Facilitates retrograde transport from the Golgi to the endoplasmic reticulum.<ref>PMID:11157978</ref> <ref>PMID:12426381</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Biterova, E I]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Guy, J E]] | + | [[Category: Biterova EI]] |
| - | [[Category: Possner, D D.D]] | + | [[Category: Guy JE]] |
| - | [[Category: Svard, M]] | + | [[Category: Possner DDD]] |
| - | [[Category: Copii vesicle]] | + | [[Category: Svard M]] |
| - | [[Category: Early secretory pathway]]
| + | |
| - | [[Category: Endoplasmic reticulum]]
| + | |
| - | [[Category: Protein transport]]
| + | |
| Structural highlights
Function
ERV41_YEAST Constituent of COPII-coated endoplasmic reticulum-derived transport vesicles. Required for efficient transport of a subset of secretory proteins to the Golgi. The C-terminal Ile-Leu motif is required for exit from the endoplasmic reticulum. Facilitates retrograde transport from the Golgi to the endoplasmic reticulum.[1] [2]
Publication Abstract from PubMed
Erv41p is a conserved integral membrane protein that is known to play a role in transport between the endoplasmic reticulum and Golgi apparatus, part of the early secretory pathway of eukaryotes. However, the exact function of the protein is not known, and it shares very low sequence identity with proteins of known structure or function. Here we present the structure of the full lumenal domain of Erv41p from Saccharomyces cerevisiae, determined by X-ray crystallography to a resolution of 2.0A. The structure reveals the protein to be composed predominantly of two large beta-sheets that form a twisted beta-sandwich. Comparison to structures in the Protein Data Bank shows that the Erv41p lumenal domain displays only limited similarity to beta-sandwich domains of other proteins. Analysis of the surface properties of the protein identifies an extensive patch of negative electrostatic potential on the exposed surface of one of the beta-sheets, which likely forms a binding site for a ligand or interaction partner. A predominantly hydrophobic region close to the membrane interface is identified as a likely site for protein-protein interaction. This structure, the first of Erv41p or any of its homologues, provides a new starting point for studies of the roles of Erv41p and its interaction partners in the eukaryotic secretory pathway.
The Crystal Structure of the Lumenal Domain of Erv41p, a Protein Involved in Transport between the Endoplasmic Reticulum and Golgi Apparatus.,Biterova EI, Svard M, Possner DD, Guy JE J Mol Biol. 2013 Mar 21. pii: S0022-2836(13)00173-3. doi:, 10.1016/j.jmb.2013.03.024. PMID:23524136[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Otte S, Belden WJ, Heidtman M, Liu J, Jensen ON, Barlowe C. Erv41p and Erv46p: new components of COPII vesicles involved in transport between the ER and Golgi complex. J Cell Biol. 2001 Feb 5;152(3):503-18. PMID:11157978
- ↑ Otte S, Barlowe C. The Erv41p-Erv46p complex: multiple export signals are required in trans for COPII-dependent transport from the ER. EMBO J. 2002 Nov 15;21(22):6095-104. PMID:12426381
- ↑ Biterova EI, Svard M, Possner DD, Guy JE. The Crystal Structure of the Lumenal Domain of Erv41p, a Protein Involved in Transport between the Endoplasmic Reticulum and Golgi Apparatus. J Mol Biol. 2013 Mar 21. pii: S0022-2836(13)00173-3. doi:, 10.1016/j.jmb.2013.03.024. PMID:23524136 doi:10.1016/j.jmb.2013.03.024
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