Structural highlights
Function
QUEFL_PYRCJ Is responsible for the final step in the biosynthesis of archaeosine, a modified nucleoside present in the dihydrouridine loop (D-loop) of archaeal tRNA (PubMed:22032275, PubMed:28383498). Catalyzes the conversion of 7-cyano-7-deazaguanine (preQ0)-modified tRNA to archaeosine-tRNA, transforming a nitrile group to a formamidine group. Can use neither glutamine nor asparagine as amino donor in vitro, is only able to utilize free ammonium (PubMed:28383498). However, the enzyme might function in vivo with a partner that serves to generate ammonium.[1] [2]
Publication Abstract from PubMed
The tunneling-fold (T-fold) structural superfamily has emerged as a versatile protein scaffold of diverse catalytic activities. This is especially evident in the pathways to the 7-deazaguanosine modified nucleosides of tRNA queuosine and archaeosine. Four members of the T-fold superfamily have been confirmed in these pathways and here we report the crystal structure of a fifth enzyme; the recently discovered amidinotransferase QueF-Like (QueF-L), responsible for the final step in the biosynthesis of archaeosine in the D-loop of tRNA in a subset of Crenarchaeota. QueF-L catalyzes the conversion of the nitrile group of the 7-cyano-7-deazaguanine (preQ0 ) base of preQ0 -modified tRNA to a formamidino group. The structure, determined in the presence of preQ0 , reveals a symmetric T-fold homodecamer of two head-to-head facing pentameric subunits, with 10 active sites at the inter-monomer interfaces. Bound preQ0 forms a stable covalent thioimide bond with a conserved active site cysteine similar to the intermediate previously observed in the nitrile reductase QueF. Despite distinct catalytic functions, phylogenetic distributions, and only 19% sequence identity, the two enzymes share a common preQ0 binding pocket, and likely a common mechanism of thioimide formation. However, due to tight twisting of its decamer, QueF-L lacks the NADPH binding site present in QueF. A large positively charged molecular surface and a docking model suggest simultaneous binding of multiple tRNA molecules and structure-specific recognition of the D-loop by a surface groove. The structure sheds light on the mechanism of nitrile amidation, and the evolution of diverse chemistries in a common fold. Proteins 2016; 85:103-116. (c) 2016 Wiley Periodicals, Inc.
Crystal structure of the archaeosine synthase QueF-like-Insights into amidino transfer and tRNA recognition by the tunnel fold.,Mei X, Alvarez J, Bon Ramos A, Samanta U, Iwata-Reuyl D, Swairjo MA Proteins. 2017 Jan;85(1):103-116. doi: 10.1002/prot.25202. Epub 2016 Nov 20. PMID:27802572[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Phillips G, Swairjo MA, Gaston KW, Bailly M, Limbach PA, Iwata-Reuyl D, de Crécy-Lagard V. Diversity of archaeosine synthesis in crenarchaeota. ACS Chem Biol. 2012 Feb 17;7(2):300-5. PMID:22032275 doi:10.1021/cb200361w
- ↑ Bon Ramos A, Bao L, Turner B, de Crécy-Lagard V, Iwata-Reuyl D. QueF-Like, a Non-Homologous Archaeosine Synthase from the Crenarchaeota. Biomolecules. 2017 Apr 6;7(2):36. PMID:28383498 doi:10.3390/biom7020036
- ↑ Mei X, Alvarez J, Bon Ramos A, Samanta U, Iwata-Reuyl D, Swairjo MA. Crystal structure of the archaeosine synthase QueF-like-Insights into amidino transfer and tRNA recognition by the tunnel fold. Proteins. 2017 Jan;85(1):103-116. doi: 10.1002/prot.25202. Epub 2016 Nov 20. PMID:27802572 doi:http://dx.doi.org/10.1002/prot.25202
