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| | <StructureSection load='5ubd' size='340' side='right'caption='[[5ubd]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='5ubd' size='340' side='right'caption='[[5ubd]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5ubd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UBD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UBD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5ubd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UBD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UBD FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.002Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5ube|5ube]], [[5ubf|5ubf]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DN30_18, EN12_14105 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ubd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ubd OCA], [https://pdbe.org/5ubd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ubd RCSB], [https://www.ebi.ac.uk/pdbsum/5ubd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ubd ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ubd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ubd OCA], [http://pdbe.org/5ubd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ubd RCSB], [http://www.ebi.ac.uk/pdbsum/5ubd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ubd ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9KNG2_VIBCH Q9KNG2_VIBCH] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillo virgola del koch trevisan 1884]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ivashkiv, O]] | |
| - | [[Category: Jeruzalmi, D]] | |
| - | [[Category: Orlova, N]] | |
| - | [[Category: Waldor, M K]] | |
| - | [[Category: Dna binding]] | |
| - | [[Category: Dna binding protein]] | |
| - | [[Category: Dna replication initiation]] | |
| - | [[Category: Secondary chromosome]] | |
| | [[Category: Vibrio cholerae]] | | [[Category: Vibrio cholerae]] |
| | + | [[Category: Ivashkiv O]] |
| | + | [[Category: Jeruzalmi D]] |
| | + | [[Category: Orlova N]] |
| | + | [[Category: Waldor MK]] |
| Structural highlights
Function
Q9KNG2_VIBCH
Publication Abstract from PubMed
The conserved DnaA-oriC system is used to initiate replication of primary chromosomes throughout the bacterial kingdom; however, bacteria with multipartite genomes evolved distinct systems to initiate replication of secondary chromosomes. In the cholera pathogen, Vibrio cholerae, and in related species, secondary chromosome replication requires the RctB initiator protein. Here, we show that RctB consists of four domains. The structure of its central two domains resembles that of several plasmid replication initiators. RctB contains at least three DNA binding winged-helix-turn-helix motifs, and mutations within any of these severely compromise biological activity. In the structure, RctB adopts a head-to-head dimeric configuration that likely reflects the arrangement in solution. Therefore, major structural reorganization likely accompanies complex formation on the head-to-tail array of binding sites in oriCII Our findings support the hypothesis that the second Vibrionaceae chromosome arose from an ancestral plasmid, and that RctB may have evolved additional regulatory features.
The replication initiator of the cholera pathogen's second chromosome shows structural similarity to plasmid initiators.,Orlova N, Gerding M, Ivashkiv O, Olinares PD, Chait BT, Waldor MK, Jeruzalmi D Nucleic Acids Res. 2016 Dec 27. pii: gkw1288. doi: 10.1093/nar/gkw1288. PMID:28031373[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Orlova N, Gerding M, Ivashkiv O, Olinares PD, Chait BT, Waldor MK, Jeruzalmi D. The replication initiator of the cholera pathogen's second chromosome shows structural similarity to plasmid initiators. Nucleic Acids Res. 2016 Dec 27. pii: gkw1288. doi: 10.1093/nar/gkw1288. PMID:28031373 doi:http://dx.doi.org/10.1093/nar/gkw1288
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