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| | <StructureSection load='5uei' size='340' side='right'caption='[[5uei]], [[Resolution|resolution]] 1.70Å' scene=''> | | <StructureSection load='5uei' size='340' side='right'caption='[[5uei]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5uei]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Petma Petma]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UEI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UEI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5uei]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Petromyzon_marinus Petromyzon marinus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UEI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UEI FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.702Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5uf1|5uf1]], [[5uf4|5uf4]], [[5ufb|5ufb]], [[5ufc|5ufc]], [[5ufd|5ufd]], [[5uff|5uff]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uei FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uei OCA], [http://pdbe.org/5uei PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uei RCSB], [http://www.ebi.ac.uk/pdbsum/5uei PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uei ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uei FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uei OCA], [https://pdbe.org/5uei PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uei RCSB], [https://www.ebi.ac.uk/pdbsum/5uei PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uei ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Variable lymphocyte receptor|Variable lymphocyte receptor]] | + | *[[Variable lymphocyte receptor 3D structures|Variable lymphocyte receptor 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| Line 25: |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Petma]] | + | [[Category: Petromyzon marinus]] |
| - | [[Category: Collins, B C]] | + | [[Category: Collins BC]] |
| - | [[Category: Cooper, M D]] | + | [[Category: Cooper MD]] |
| - | [[Category: Cummings, R D]] | + | [[Category: Cummings RD]] |
| - | [[Category: Gunn, R J]] | + | [[Category: Gunn RJ]] |
| - | [[Category: Herrin, B R]] | + | [[Category: Herrin BR]] |
| - | [[Category: McKitrick, T R]] | + | [[Category: McKitrick TR]] |
| - | [[Category: Wilson, I A]] | + | [[Category: Wilson IA]] |
| - | [[Category: Adaptive immunity]]
| + | |
| - | [[Category: Glycan binding]]
| + | |
| - | [[Category: Glycan receptor]]
| + | |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Jawless fish]]
| + | |
| - | [[Category: Leucine-rich repeat]]
| + | |
| - | [[Category: Lrr]]
| + | |
| - | [[Category: Receptor]]
| + | |
| - | [[Category: Sea lamprey]]
| + | |
| - | [[Category: Variable lymphocyte receptor]]
| + | |
| - | [[Category: Vlr]]
| + | |
| Structural highlights
Publication Abstract from PubMed
High-quality reagents to study and detect glycans with high specificity for research and clinical applications are severely lacking. Here, we structurally and functionally characterize several variable lymphocyte receptor (VLR)-based antibodies from lampreys immunized with O erythrocytes that specifically recognize the blood group H-trisaccharide type II antigen. Glycan microarray analysis and biophysical data reveal that these VLRs exhibit greater specificity for H-trisaccharide compared with the plant lectin UEA-1, which is widely used in blood typing. Among these antibodies, O13 exhibits superior specificity for H-trisaccharide, the basis for which is revealed by comparative analysis of high-resolution VLR:glycan crystal structures. Using a structure-guided approach, we designed an O13 mutant with further enhanced specificity for H-trisaccharide. These insights into glycan recognition by VLRs suggest that lampreys can produce highly specific glycan antibodies, and are a valuable resource for the production of next-generation glycan reagents for biological and biomedical research and as diagnostics and therapeutics.
Structural Insights into VLR Fine Specificity for Blood Group Carbohydrates.,Collins BC, Gunn RJ, McKitrick TR, Cummings RD, Cooper MD, Herrin BR, Wilson IA Structure. 2017 Sep 27. pii: S0969-2126(17)30294-0. doi:, 10.1016/j.str.2017.09.003. PMID:28988747[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Collins BC, Gunn RJ, McKitrick TR, Cummings RD, Cooper MD, Herrin BR, Wilson IA. Structural Insights into VLR Fine Specificity for Blood Group Carbohydrates. Structure. 2017 Sep 27. pii: S0969-2126(17)30294-0. doi:, 10.1016/j.str.2017.09.003. PMID:28988747 doi:http://dx.doi.org/10.1016/j.str.2017.09.003
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