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| | <StructureSection load='5v8w' size='340' side='right'caption='[[5v8w]], [[Resolution|resolution]] 2.10Å' scene=''> | | <StructureSection load='5v8w' size='340' side='right'caption='[[5v8w]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5v8w]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V8W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5V8W FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5v8w]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V8W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5V8W FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">INTS9, RC74 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CPSF3L, INTS11, RC68 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5v8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5v8w OCA], [http://pdbe.org/5v8w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5v8w RCSB], [http://www.ebi.ac.uk/pdbsum/5v8w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5v8w ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5v8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5v8w OCA], [https://pdbe.org/5v8w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5v8w RCSB], [https://www.ebi.ac.uk/pdbsum/5v8w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5v8w ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/INT9_HUMAN INT9_HUMAN]] Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. [[http://www.uniprot.org/uniprot/INT11_HUMAN INT11_HUMAN]] Catalytic component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates the snRNAs 3' cleavage.<ref>PMID:16239144</ref> | + | [https://www.uniprot.org/uniprot/INT9_HUMAN INT9_HUMAN] Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 5v8w" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5v8w" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Integrator complex 3D structures|Integrator complex 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Tong, L]] | + | [[Category: Tong L]] |
| - | [[Category: Wu, Y]] | + | [[Category: Wu Y]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Integrator complex]]
| + | |
| - | [[Category: Snrna processing]]
| + | |
| Structural highlights
Function
INT9_HUMAN Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.
Publication Abstract from PubMed
The metazoan Integrator complex (INT) has important functions in the 3'-end processing of noncoding RNAs, including the uridine-rich small nuclear RNA (UsnRNA) and enhancer RNA (eRNA), and in the transcription of coding genes by RNA polymerase II. The INT contains at least 14 subunits, but its molecular mechanism of action is poorly understood, because currently there is little structural information about its subunits. The endonuclease activity of INT is mediated by its subunit 11 (IntS11), which belongs to the metallo-beta-lactamase superfamily and is a paralog of CPSF-73, the endonuclease for pre-mRNA 3'-end processing. IntS11 forms a stable complex with Integrator complex subunit 9 (IntS9) through their C-terminal domains (CTDs). Here, we report the crystal structure of the IntS9-IntS11 CTD complex at 2.1-A resolution and detailed, structure-based biochemical and functional studies. The complex is composed of a continuous nine-stranded beta-sheet with four strands from IntS9 and five from IntS11. Highly conserved residues are located in the extensive interface between the two CTDs. Yeast two-hybrid assays and coimmunoprecipitation experiments confirm the structural observations on the complex. Functional studies demonstrate that the IntS9-IntS11 interaction is crucial for the role of INT in snRNA 3'-end processing.
Molecular basis for the interaction between Integrator subunits IntS9 and IntS11 and its functional importance.,Wu Y, Albrecht TR, Baillat D, Wagner EJ, Tong L Proc Natl Acad Sci U S A. 2017 Apr 25;114(17):4394-4399. doi:, 10.1073/pnas.1616605114. Epub 2017 Apr 10. PMID:28396433[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Wu Y, Albrecht TR, Baillat D, Wagner EJ, Tong L. Molecular basis for the interaction between Integrator subunits IntS9 and IntS11 and its functional importance. Proc Natl Acad Sci U S A. 2017 Apr 25;114(17):4394-4399. doi:, 10.1073/pnas.1616605114. Epub 2017 Apr 10. PMID:28396433 doi:http://dx.doi.org/10.1073/pnas.1616605114
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