|
|
| Line 3: |
Line 3: |
| | <SX load='5z3g' size='340' side='right' viewer='molstar' caption='[[5z3g]], [[Resolution|resolution]] 3.65Å' scene=''> | | <SX load='5z3g' size='340' side='right' viewer='molstar' caption='[[5z3g]], [[Resolution|resolution]] 3.65Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5z3g]] is a 35 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z3G OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5Z3G FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5z3g]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z3G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Z3G FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.65Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5z3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z3g OCA], [https://pdbe.org/5z3g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5z3g RCSB], [https://www.ebi.ac.uk/pdbsum/5z3g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5z3g ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5z3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z3g OCA], [http://pdbe.org/5z3g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z3g RCSB], [http://www.ebi.ac.uk/pdbsum/5z3g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z3g ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RL4A_YEAST RL4A_YEAST]] Participates in the regulation of the accumulation of its own mRNA.<ref>PMID:2065661</ref> [[http://www.uniprot.org/uniprot/MAK16_YEAST MAK16_YEAST]] Its role might be as part of the apparatus concerned with the nuclear events of the cell cycle.<ref>PMID:3045810</ref> [[http://www.uniprot.org/uniprot/BRX1_YEAST BRX1_YEAST]] Required for biogenesis of the 60S ribosomal subunit.<ref>PMID:11843177</ref> [[http://www.uniprot.org/uniprot/HAS1_YEAST HAS1_YEAST]] ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA.<ref>PMID:15049817</ref> <ref>PMID:15242642</ref> <ref>PMID:15718299</ref> [[http://www.uniprot.org/uniprot/EBP2_YEAST EBP2_YEAST]] Required for the processing of the 27S pre-rRNA. Probably involved in the step of the processing of the 27 SA precursor into the 27 SB intermediate.<ref>PMID:10849420</ref> <ref>PMID:10947841</ref> [[http://www.uniprot.org/uniprot/IF6_YEAST IF6_YEAST]] Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by the GTPase RIA1/EFL1 and by SDO1. Also required for pre-rRNA processing.<ref>PMID:10085284</ref> <ref>PMID:11779510</ref> <ref>PMID:11238882</ref> <ref>PMID:17353896</ref> <ref>PMID:18256024</ref> <ref>PMID:11101899</ref> [[http://www.uniprot.org/uniprot/RRP1_YEAST RRP1_YEAST]] Required for 27S rRNA processing to 25S and 5.8S. [[http://www.uniprot.org/uniprot/RL37A_YEAST RL37A_YEAST]] Binds to the 23S rRNA (By similarity). [[http://www.uniprot.org/uniprot/NSA1_YEAST NSA1_YEAST]] Involved in the biogenesis of the 60S ribosomal subunit.<ref>PMID:16544271</ref> [[http://www.uniprot.org/uniprot/PESC_YEAST PESC_YEAST]] Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.[HAMAP-Rule:MF_03028]<ref>PMID:11911362</ref> <ref>PMID:12022229</ref> <ref>PMID:12110181</ref> <ref>PMID:18448671</ref> [[http://www.uniprot.org/uniprot/RPF1_YEAST RPF1_YEAST]] Essential protein. Required for biogenesis of the 60S ribosomal subunit.<ref>PMID:11864606</ref> <ref>PMID:12702244</ref> [[http://www.uniprot.org/uniprot/CIC1_YEAST CIC1_YEAST]] An adapter protein that specifically links the 26S proteasome to its substrate CDC4 which is one of the substrate recognition subunits of the SCF E3 ubiquitin ligase complex. Required for turnover of cell cycle regulatory proteins CDC4 and GRR1. Required for synthesis and nuclear export of 60S ribosomal subunits. Required for vegetative growth.<ref>PMID:11500370</ref> <ref>PMID:14623999</ref> [[http://www.uniprot.org/uniprot/RLP7_YEAST RLP7_YEAST]] Involved in the biogenesis of the 60S ribosomal subunit. May act as a specificity factor that binds precursor rRNAs and tethers the enzymes that carry out the early 5' to 3' exonucleolytic reactions that generate the mature rRNAs.<ref>PMID:11087857</ref> [[http://www.uniprot.org/uniprot/NOP15_YEAST NOP15_YEAST]] Involved in the biogenesis of the 60S ribosomal subunit. Required for pre-rRNA processing and cytokinesis. Associates with the precursors of the 25S and 5.8S rRNAs.<ref>PMID:11583614</ref> <ref>PMID:14657029</ref> | + | [https://www.uniprot.org/uniprot/RL6A_YEAST RL6A_YEAST] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 22: |
Line 21: |
| | ==See Also== | | ==See Also== |
| | *[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]] | | *[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]] |
| | + | *[[Helicase 3D structures|Helicase 3D structures]] |
| | *[[Ribosome 3D structures|Ribosome 3D structures]] | | *[[Ribosome 3D structures|Ribosome 3D structures]] |
| - | *[[Ribosome biogenesis protein|Ribosome biogenesis protein]] | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| Line 29: |
Line 28: |
| | </SX> | | </SX> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: RNA helicase]] | |
| | [[Category: Saccharomyces cerevisiae]] | | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Saccharomyces cerevisiae s288c]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Ye, K]] | + | [[Category: Ye K]] |
| - | [[Category: Zhou, D]] | + | [[Category: Zhou D]] |
| - | [[Category: Zhu, X]] | + | [[Category: Zhu X]] |
| - | [[Category: Pre-60]]
| + | |
| - | [[Category: Pre-ribosome]]
| + | |
| - | [[Category: Protein-rna complex]]
| + | |
| - | [[Category: Ribosome]]
| + | |
| Structural highlights
Function
RL6A_YEAST
Publication Abstract from PubMed
Assembly of eukaryotic ribosome is a complicated and dynamic process that involves a series of intermediates. It is unknown how the highly intertwined structure of 60S large ribosomal subunits is established. Here, we report the structure of an early nucleolar pre-60S ribosome determined by cryo-electron microscopy at 3.7 A resolution, revealing a half-assembled subunit. Domains I, II and VI of 25S/5.8S rRNA pack tightly into a native-like substructure, but domains III, IV and V are not assembled. The structure contains 12 assembly factors and 19 ribosomal proteins, many of which are required for early processing of large subunit rRNA. The Brx1-Ebp2 complex would interfere with the assembly of domains IV and V. Rpf1, Mak16, Nsa1 and Rrp1 form a cluster that consolidates the joining of domains I and II. Our structure reveals a key intermediate on the path to establishing the global architecture of 60S subunits.
Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate.,Zhou D, Zhu X, Zheng S, Tan D, Dong MQ, Ye K Protein Cell. 2018 Mar 19. pii: 10.1007/s13238-018-0526-7. doi:, 10.1007/s13238-018-0526-7. PMID:29557065[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Zhou D, Zhu X, Zheng S, Tan D, Dong MQ, Ye K. Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate. Protein Cell. 2018 Mar 19. pii: 10.1007/s13238-018-0526-7. doi:, 10.1007/s13238-018-0526-7. PMID:29557065 doi:http://dx.doi.org/10.1007/s13238-018-0526-7
|
