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| | ==NMR structure of IRD12 from Capsicum annum.== | | ==NMR structure of IRD12 from Capsicum annum.== |
| - | <StructureSection load='5zfo' size='340' side='right'caption='[[5zfo]], [[NMR_Ensembles_of_Models | 11 NMR models]]' scene=''> | + | <StructureSection load='5zfo' size='340' side='right'caption='[[5zfo]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5zfo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Capan Capan]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZFO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZFO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5zfo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Capsicum_annuum Capsicum annuum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZFO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZFO FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zfo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zfo OCA], [http://pdbe.org/5zfo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zfo RCSB], [http://www.ebi.ac.uk/pdbsum/5zfo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zfo ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 11 models</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zfo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zfo OCA], [https://pdbe.org/5zfo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zfo RCSB], [https://www.ebi.ac.uk/pdbsum/5zfo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zfo ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/T1PZ86_CAPAN T1PZ86_CAPAN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Capan]] | + | [[Category: Capsicum annuum]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Barnwal, R P]] | + | [[Category: Barnwal RP]] |
| - | [[Category: Chary, K V.R]] | + | [[Category: Chary KVR]] |
| - | [[Category: Gartia, J]] | + | [[Category: Gartia J]] |
| - | [[Category: Capsicum annum]]
| + | |
| - | [[Category: Inhibitor]]
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| - | [[Category: Plant protein]]
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| - | [[Category: Protein]]
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| Structural highlights
Function
T1PZ86_CAPAN
Publication Abstract from PubMed
Although several plant protease inhibitors have been structurally characterized using X-ray crystallography, very few have been studied using NMR techniques. Here, we report an NMR study of the solution structure and dynamics of an inhibitory repeat domain (IRD) variant 12 from the wound-inducible Pin-II type proteinase inhibitor from Capsicum annuum. IRD variant 12 (IRD12) showed strong anti-metabolic activity against the Lepidopteran insect pest, Helicoverpa armigera. The NMR-derived three-dimensional structure of IRD12 reveals a three-stranded anti-parallel beta-sheet rigidly held together by four disulfide bridges and shows structural homology with known IRDs. It is interesting to note that the IRD12 structure containing approximately 75% unstructured part still shows substantial amount of rigidity of N-H bond vectors with respect to its molecular motion. Communicated by Ramaswamy H. Sarma.
NMR structure and dynamics of inhibitory repeat domain variant 12, a plant protease inhibitor from Capsicum annuum, and its structural relationship to other plant protease inhibitors.,Gartia J, Anangi R, Joshi RS, Giri AP, King GF, Barnwal RP, Chary KVR J Biomol Struct Dyn. 2019 Apr 30:1-10. doi: 10.1080/07391102.2019.1607559. PMID:31038412[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gartia J, Anangi R, Joshi RS, Giri AP, King GF, Barnwal RP, Chary KVR. NMR structure and dynamics of inhibitory repeat domain variant 12, a plant protease inhibitor from Capsicum annuum, and its structural relationship to other plant protease inhibitors. J Biomol Struct Dyn. 2019 Apr 30:1-10. doi: 10.1080/07391102.2019.1607559. PMID:31038412 doi:http://dx.doi.org/10.1080/07391102.2019.1607559
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