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| | ==Crystal structure of the C-terminal periplasmic domain of EcEptC from Escherichia coli== | | ==Crystal structure of the C-terminal periplasmic domain of EcEptC from Escherichia coli== |
| - | <StructureSection load='6a82' size='340' side='right' caption='[[6a82]], [[Resolution|resolution]] 2.10Å' scene=''> | + | <StructureSection load='6a82' size='340' side='right'caption='[[6a82]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6a82]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A82 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A82 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6a82]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A82 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6A82 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">eptC, cptA, yijP, b3955, JW3927 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6a82 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a82 OCA], [https://pdbe.org/6a82 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6a82 RCSB], [https://www.ebi.ac.uk/pdbsum/6a82 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6a82 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6a82 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a82 OCA], [http://pdbe.org/6a82 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a82 RCSB], [http://www.ebi.ac.uk/pdbsum/6a82 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a82 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/EPTC_ECOLI EPTC_ECOLI]] Catalyzes the addition of a phosphoethanolamine moiety to the outer membrane lipopolysaccharide core. | + | [https://www.uniprot.org/uniprot/EPTC_ECOLI EPTC_ECOLI] Catalyzes the addition of a phosphoethanolamine moiety to the outer membrane lipopolysaccharide core. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ecoli]] | + | [[Category: Escherichia coli K-12]] |
| - | [[Category: Cheng, W]] | + | [[Category: Large Structures]] |
| - | [[Category: Gu, Y J]] | + | [[Category: Cheng W]] |
| - | [[Category: Zhao, Y Q]] | + | [[Category: Gu YJ]] |
| - | [[Category: Phosphoethanolamine transferase]] | + | [[Category: Zhao YQ]] |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
EPTC_ECOLI Catalyzes the addition of a phosphoethanolamine moiety to the outer membrane lipopolysaccharide core.
Publication Abstract from PubMed
Gram-negative bacteria defend against the toxicity of polymyxins by modifying their outer membrane lipopolysaccharide (LPS). This modification mainly occurs through the addition of cationic molecules such as phosphoethanolamine (PEA). EcEptC is a PEA transferase from Escherichia coli (E. coli). However, unlike its homologs CjEptC (Campylobacter jejuni) and MCR-1, EcEptC is unable to mediate polymyxin resistance when overexpressed in E. coli. Here, we report crystal structures of the C-terminal putative catalytic domain (EcEptCDeltaN, 205-577 aa) of EcEptC in apo and Zn(2+) -bound states at 2.10 and 2.60 A, respectively. EcEptCDeltaN is arranged into an alpha-beta-alpha fold and equipped with the zinc ion in a conserved mode. Coupled with isothermal titration calorimetry (ITC) data, we provide insights into the mechanism by which EcEptC recognizes Zn(2+) . Furthermore, structure comparison analysis indicated that disulfide bonds, which play a key role in polymyxin resistance, were absent in EcEptCDeltaN. Supported by structural and biochemical evidence, we reveal mechanistic implications for disulfide bonds in PEA transferase-mediated polymyxin resistance. Significantly, because the structural effects exhibited by disulfide bonds are absent in EcEptC, it is impossible for this protein to participate in polymyxin resistance in E. coli. DATABASE: Structural data are available in the PDB under the accession numbers 6A82 and 6A83. ENZYME: EC 2.7.8.43.
Structural and mechanistic insights into polymyxin resistance mediated by EptC originating from Escherichia coli.,Zhao Y, Meng Q, Lai Y, Wang L, Zhou D, Dou C, Gu Y, Nie C, Wei Y, Cheng W FEBS J. 2018 Dec 7. doi: 10.1111/febs.14719. PMID:30537137[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zhao Y, Meng Q, Lai Y, Wang L, Zhou D, Dou C, Gu Y, Nie C, Wei Y, Cheng W. Structural and mechanistic insights into polymyxin resistance mediated by EptC originating from Escherichia coli. FEBS J. 2018 Dec 7. doi: 10.1111/febs.14719. PMID:30537137 doi:http://dx.doi.org/10.1111/febs.14719
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