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| | <StructureSection load='6eez' size='340' side='right'caption='[[6eez]], [[Resolution|resolution]] 2.25Å' scene=''> | | <StructureSection load='6eez' size='340' side='right'caption='[[6eez]], [[Resolution|resolution]] 2.25Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6eez]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Wolpm Wolpm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EEZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EEZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6eez]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Wolbachia_endosymbiont_of_Drosophila_melanogaster Wolbachia endosymbiont of Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EEZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6EEZ FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">WD_1312 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=163164 WOLPM])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6eez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eez OCA], [http://pdbe.org/6eez PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eez RCSB], [http://www.ebi.ac.uk/pdbsum/6eez PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eez ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6eez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eez OCA], [https://pdbe.org/6eez PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6eez RCSB], [https://www.ebi.ac.uk/pdbsum/6eez PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6eez ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q73FL6_WOLPM Q73FL6_WOLPM] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Wolpm]] | + | [[Category: Wolbachia endosymbiont of Drosophila melanogaster]] |
| - | [[Category: King, G J]] | + | [[Category: King GJ]] |
| - | [[Category: Lakshmanane, P]] | + | [[Category: Lakshmanane P]] |
| - | [[Category: Martin, J L]] | + | [[Category: Martin JL]] |
| - | [[Category: Walden, P M]] | + | [[Category: Walden PM]] |
| - | [[Category: Isomerase]]
| + | |
| - | [[Category: Thiol-disulfide exchange protein]]
| + | |
| Structural highlights
Function
Q73FL6_WOLPM
Publication Abstract from PubMed
Disulfide-bond-forming (DSB) oxidative folding enzymes are master regulators of virulence that are localized to the periplasm of many Gram-negative bacteria. The archetypal DSB machinery from Escherichia coli K-12 consists of a dithiol-oxidizing redox-relay pair (DsbA/B), a disulfide-isomerizing redox-relay pair (DsbC/D) and the specialist reducing enzymes DsbE and DsbG that also interact with DsbD. By contrast, the Gram-negative bacterium Wolbachia pipientis encodes just three DSB enzymes. Two of these, alpha-DsbA1 and alpha-DsbB, form a redox-relay pair analogous to DsbA/B from E. coli. The third enzyme, alpha-DsbA2, incorporates a DsbA-like sequence but does not interact with alpha-DsbB. In comparison to other DsbA enzymes, alpha-DsbA2 has approximately 50 extra N-terminal residues (excluding the signal peptide). The crystal structure of alpha-DsbA2DeltaN, an N-terminally truncated form in which these approximately 50 residues are removed, confirms the DsbA-like nature of this domain. However, alpha-DsbA2 does not have DsbA-like activity: it is structurally and functionally different as a consequence of its N-terminal residues. Firstly, alpha-DsbA2 is a powerful disulfide isomerase and a poor dithiol oxidase: i.e. its role is to shuffle rather than to introduce disulfide bonds. Moreover, small-angle X-ray scattering (SAXS) of alpha-DsbA2 reveals a homotrimeric arrangement that differs from those of the other characterized bacterial disulfide isomerases DsbC from Escherichia coli (homodimeric) and ScsC from Proteus mirabilis (PmScsC; homotrimeric with a shape-shifter peptide). alpha-DsbA2 lacks the shape-shifter motif and SAXS data suggest that it is less flexible than PmScsC. These results allow conclusions to be drawn about the factors that are required for functionally equivalent disulfide isomerase enzymatic activity across structurally diverse protein architectures.
The atypical thiol-disulfide exchange protein alpha-DsbA2 from Wolbachia pipientis is a homotrimeric disulfide isomerase.,Walden PM, Whitten AE, Premkumar L, Halili MA, Heras B, King GJ, Martin JL Acta Crystallogr D Struct Biol. 2019 Mar 1;75(Pt 3):283-295. doi:, 10.1107/S2059798318018442. Epub 2019 Feb 26. PMID:30950399[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Walden PM, Whitten AE, Premkumar L, Halili MA, Heras B, King GJ, Martin JL. The atypical thiol-disulfide exchange protein alpha-DsbA2 from Wolbachia pipientis is a homotrimeric disulfide isomerase. Acta Crystallogr D Struct Biol. 2019 Mar 1;75(Pt 3):283-295. doi:, 10.1107/S2059798318018442. Epub 2019 Feb 26. PMID:30950399 doi:http://dx.doi.org/10.1107/S2059798318018442
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