7bhp
From Proteopedia
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| - | ==== | + | ==Cryo-EM structure of the human Ebp1 - 80S ribosome== |
| - | <StructureSection load='7bhp' size='340' side='right'caption='[[7bhp]]' scene=''> | + | <StructureSection load='7bhp' size='340' side='right'caption='[[7bhp]], [[Resolution|resolution]] 3.30Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7bhp]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BHP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BHP FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bhp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bhp OCA], [https://pdbe.org/7bhp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bhp RCSB], [https://www.ebi.ac.uk/pdbsum/7bhp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bhp ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bhp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bhp OCA], [https://pdbe.org/7bhp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bhp RCSB], [https://www.ebi.ac.uk/pdbsum/7bhp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bhp ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/RL8_HUMAN RL8_HUMAN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Ribosomes are the macromolecular machines at the heart of protein synthesis; however, their function can be modulated by a variety of additional protein factors that directly interact with them. Here, we report the cryo-EM structure of human Ebp1 (p48 isoform) bound to the human 80S ribosome at 3.3 A resolution. Ebp1 binds in the vicinity of the peptide exit tunnel on the 80S ribosome, and this binding is enhanced upon puromycin-mediated translational inhibition. The association of Ebp1 with the 80S ribosome centers around its interaction with ribosomal proteins eL19 and uL23 and the 28S rRNA. Further analysis of the Ebp1-ribosome complex suggests that Ebp1 can rotate around its insert domain, which may enable it to assume a wide range of conformations while maintaining its interaction with the ribosome. Structurally, Ebp1 shares homology with the methionine aminopeptidase 2 family of enzymes; therefore, this inherent flexibility may also be conserved. | ||
| + | |||
| + | Dynamic association of human Ebp1 with the ribosome.,Bhaskar V, Desogus J, Graff-Meyer A, Schenk AD, Cavadini S, Chao JA RNA. 2021 Apr;27(4):411-419. doi: 10.1261/rna.077602.120. Epub 2021 Jan 21. PMID:33479117<ref>PMID:33479117</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7bhp" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Ribosome 3D structures|Ribosome 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Bhaskar V]] |
| + | [[Category: Chao JA]] | ||
| + | [[Category: Desogus J]] | ||
Current revision
Cryo-EM structure of the human Ebp1 - 80S ribosome
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