7t2r
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | ==== | + | ==Structure of electron bifurcating Ni-Fe hydrogenase complex HydABCSL in FMN-free apo state== |
| - | <StructureSection load='7t2r' size='340' side='right'caption='[[7t2r]]' scene=''> | + | <StructureSection load='7t2r' size='340' side='right'caption='[[7t2r]], [[Resolution|resolution]] 3.20Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7t2r]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Acetomicrobium_mobile Acetomicrobium mobile]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T2R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T2R FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t2r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t2r OCA], [https://pdbe.org/7t2r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t2r RCSB], [https://www.ebi.ac.uk/pdbsum/7t2r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t2r ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3NI:NICKEL+(III)+ION'>3NI</scene>, <scene name='pdbligand=FCO:CARBONMONOXIDE-(DICYANO)+IRON'>FCO</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t2r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t2r OCA], [https://pdbe.org/7t2r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t2r RCSB], [https://www.ebi.ac.uk/pdbsum/7t2r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t2r ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/I4BYB4_ACEMN I4BYB4_ACEMN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Electron bifurcation enables thermodynamically unfavorable biochemical reactions. Four groups of bifurcating flavoenzyme are known and three use FAD to bifurcate. FeFe-HydABC hydrogenase represents the fourth group, but its bifurcation site is unknown. We report cryo-EM structures of the related NiFe-HydABCSL hydrogenase that reversibly oxidizes H(2) and couples endergonic reduction of ferredoxin with exergonic reduction of NAD. FMN surrounded by a unique arrangement of iron sulfur clusters forms the bifurcating center. NAD binds to FMN in HydB, and electrons from H(2) via HydA to a HydB [4Fe-4S] cluster enable the FMN to reduce NAD. Low-potential electron transfer from FMN to the HydC [2Fe-2S] cluster and subsequent reduction of a uniquely penta-coordinated HydB [2Fe-2S] cluster require conformational changes, leading to ferredoxin binding and reduction by a [4Fe-4S] cluster in HydB. This work clarifies the electron transfer pathways for a large group of hydrogenases underlying many essential functions in anaerobic microorganisms. | ||
| + | |||
| + | Structure and electron transfer pathways of an electron-bifurcating NiFe-hydrogenase.,Feng X, Schut GJ, Haja DK, Adams MWW, Li H Sci Adv. 2022 Feb 25;8(8):eabm7546. doi: 10.1126/sciadv.abm7546. Epub 2022 Feb , 25. PMID:35213221<ref>PMID:35213221</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7t2r" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Acetomicrobium mobile]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Feng X]] |
| + | [[Category: Li H]] | ||
Current revision
Structure of electron bifurcating Ni-Fe hydrogenase complex HydABCSL in FMN-free apo state
| |||||||||||
