7z14
From Proteopedia
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==Cryo-EM structure of Torpedo nicotinic acetylcholine receptor in complex with a short-chain neurotoxin.== | ==Cryo-EM structure of Torpedo nicotinic acetylcholine receptor in complex with a short-chain neurotoxin.== | ||
| - | <StructureSection load='7z14' size='340' side='right'caption='[[7z14]]' scene=''> | + | <StructureSection load='7z14' size='340' side='right'caption='[[7z14]], [[Resolution|resolution]] 3.15Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z14 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z14 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7z14]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetronarce_californica Tetronarce californica] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z14 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z14 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z14 OCA], [https://pdbe.org/7z14 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z14 RCSB], [https://www.ebi.ac.uk/pdbsum/7z14 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z14 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.15Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z14 OCA], [https://pdbe.org/7z14 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z14 RCSB], [https://www.ebi.ac.uk/pdbsum/7z14 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z14 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/ACHA_TETCF ACHA_TETCF] After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bites by elapid snakes (e.g. cobras) can result in life-threatening paralysis caused by venom neurotoxins blocking neuromuscular nicotinic acetylcholine receptors. Here, we determine the cryo-EM structure of the muscle-type Torpedo receptor in complex with ScNtx, a recombinant short-chain alpha-neurotoxin. ScNtx is pinched between loop C on the principal subunit and a unique hairpin in loop F on the complementary subunit, thereby blocking access to the neurotransmitter binding site. ScNtx adopts a binding mode that is tilted toward the complementary subunit, forming a wider network of interactions than those seen in the long-chain alpha-Bungarotoxin complex. Certain mutations in ScNtx at the toxin-receptor interface eliminate inhibition of neuronal alpha7 nAChRs, but not of human muscle-type receptors. These observations explain why ScNtx binds more tightly to muscle-type receptors than neuronal receptors. Together, these data offer a framework for understanding subtype-specific actions of short-chain alpha-neurotoxins and inspire strategies for design of new snake antivenoms. | ||
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| + | The molecular mechanism of snake short-chain alpha-neurotoxin binding to muscle-type nicotinic acetylcholine receptors.,Nys M, Zarkadas E, Brams M, Mehregan A, Kambara K, Kool J, Casewell NR, Bertrand D, Baenziger JE, Nury H, Ulens C Nat Commun. 2022 Aug 4;13(1):4543. doi: 10.1038/s41467-022-32174-7. PMID:35927270<ref>PMID:35927270</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7z14" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Acetyl choline receptor 3D structures|Acetyl choline receptor 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Tetronarce californica]] | ||
[[Category: Nury H]] | [[Category: Nury H]] | ||
[[Category: Nys MAEM]] | [[Category: Nys MAEM]] | ||
[[Category: Ulens C]] | [[Category: Ulens C]] | ||
[[Category: Zarkadas E]] | [[Category: Zarkadas E]] | ||
Current revision
Cryo-EM structure of Torpedo nicotinic acetylcholine receptor in complex with a short-chain neurotoxin.
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